A novel injectable hydrogel based on DNA and silicate nanodisks was fabricated and optimized to obtain a suitable drug delivery platform for biomedical applications. Precisely, the hydrogel was designed by combining two different type of networks: a first network (type A) made of interconnections between neighboring DNA strands and a second one (type B) consisting of electrostatic interactions between the silicate nanodisks and the DNA backbone. The silicate nanodisks were introduced to increase the viscosity of the DNA physical hydrogel and improve their shear-thinning properties. Additionally, the silicate nanodisks were selected to modulate the release capability of the designed network.

DNA 4% solutions were heated at 90°C for 45 seconds and cooled down at 37°C degree for two hours. In the second step, the silicate nanodisks suspension in water at different concentrations (0.1 up to 0.5%) were then mixed with the pre-gel DNA hydrogels to obtain the nanocomposite hydrogels. Rheological studies were carried out to investigate the shear thinning properties of the hydrogels. Additionally, the hydrogels were characterized by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron microscopy. The hydrogels were loaded with the osteoinductive drug dexamethasone and its release was tested in vitro in phosphate buffer pH 7.4. The drug activity upon release was tested evaluating the osteogenic differentiation of human adipose derived stem cells (hASCs) in vitro through analysis of main osteogenic markers and quantification of alkaline phosphatase activity and calcium deposition. Finally, the hydrogels were tested in vivo and injected into cranial defects in rats to assess their biocompatibility and bone regeneration potential.

The inclusion of the silicate nanodisks increased the viscosity of the hydrogels and the best results were obtained with the highest concentration of the nanoclay (0.5%). The hydrogels possessed shear-thinning properties as demonstrated by cyclic strain sweep tests and were able to recover their original storage modulus G' upon removal of strain. Such improvement in the injectable properties of the formulated hydrogels was mainly attributed to the formation of electrostatic interactions between the silicate nanodisks and the phosphate groups of the DNA backbone as confirmed by XPS analysis of the O, N, and P spectra. Additionally, laponite was able to sustain the release of the osteoinductive drug dexamethasone which was instead completely released from the DNA-based hydrogels after a week. The drug after being released was still active and promoted the osteogenic differentiation of hASCs as confirmed by ALP expression and expression of main osteogenic markers including ALP and COLA1. Finally, the gels proved to be biocompatible in vivo when injected into cranial defects and promoted bone formation at the periphery of the defect after a month post-treatment.

A novel injectable shear-thinning DNA-based hydrogel was characterized and tested for its drug delivery properties. The hydrogel can promote the sustain release of a small molecule like dexamethasone and be biocompatible in vitro and in vivo. Due to these promising findings, the designed system could find also applicability for the delivery of growth factors or other therapeutic molecules.

Introduction

Long-term success of the cementless acetabular component has been depends on amount of bone ingrowth around porous coated surface of the implant, which is mainly depends on primary stability, i.e. amount of micromotion at the implant-bone interface. The accurate positioning of the uncemented acetabular component and amount of interference fit (press-fit) at the rim of the acetabulum are necessary to reduce the implant-bone micromotion and that can be enhancing the bone ingrowth around the uncemented acetabular component. However, the effect of implant orientations and amount of press-fit on implant-bone micromotion around uncemented acetabular component has been relatively under investigated. The aim of the study is to identify the effect of acetabular component orientation on implant-bone relative micromotion around cementless metallic acetabular component.

Background

Although soft tissue sarcoma (STS) is a rare malignancy, myxofibrosarcoma is a common form diagnosed. Myxofibrosarcoma is complicated by a high local recurrence rate (18–54%) and significant morbidity following treatment, hence management can be challenging.

Haemangiomas are benign tumours with increased number of normal or abnormal appearing blood vessels. They are the commonest soft tissue tumours of infancy and childhood and comprise 7% of all soft tissue tumours.

Our study is a retrospective analysis of 120 referred cases of various vascular anomalies in the last 10 years. Eighty cases had confirmed haemangiomas. MRI scan and needle biopsy formed the basis of diagnosis. M:F = 42:38. Mean age at presentation was 34.8 years, with the youngest and eldest patient being 3.5 and 78 years respectively. 5 patients were lost to study. Sites of occurrence were upper limb(32), lower limb(32), axilla(3), foot(5), thumb(1), knee(4), spine(1), posterior chest wall(2). 55/80 patients were managed non-operatively by way of Sclerotherapy/Embolisation, watchful observation or symptomatic treatment. 4/55 cases were assessed to be unsuitable for sclerotherapy and 1 patient was subjected to surgery. 6/55 cases did not respond to sclerotherapy. 25/55 cases were managed with surgical excision. Complete excision was the primary goal of surgery. Intralesional margins were accepted if lesions were close to neurovascular structures. Indications for surgery were 1) Pain with functional and/or developmental disturbance, 2) Sudden increase in size, 3) Recurrent haemarthrosis, 4) Failure of sclerotherapy. There were 3 cases of incomplete excision among those operated. 6/25 cases had recurrence 1-8 years after surgery. Mean follow-up was 38.4 weeks (range6-12 months). We have had a success rate of 81.48% with non-operative management of symptomatic haemangiomas. Surgical excision of haemangiomas has borne 76% satisfactory results for pain relief/functional recovery.

We believe that extensive haemangiomata covering large surface areas are not suitable for surgical excision. Majority of cases can be successfully managed non-operatively by way of sclerotherapy/embolisation and watchful observation. MRI scan coupled with trucut needle biopsy has been most successful for diagnosis. Highly vascular sarcomata can mimic haemangiomas, hence histological diagnosis is crucial.

Background: The literature on description and management of advanced fungating soft tissue tumours (FSST) is limited because of the rarity of cases. Recent advances in diagnostic resources and an increased awareness of the disease has made early recognition easier. Manchester Royal Infirmary is a Regional Sarcoma Centre in the North West of England. We describe our experiences in managing patients with FSST of the extremities.

Patients and Methods: Between 1997 and 2007, 18 patients presented with FSST of the extremities (13 involving the lower limb, and 5 involving the upper limb), and 1 patient with a sarcoma involving the scapular region (limb girdle). The cohort included 14 males and 5 females with a mean average age of 68.5 ± 13.7 years. Follow-up ranged from a minimum of 6 months to 10 years from the initial referral.

Results: The histological diagnosis was sarcoma in 15 patients, subclassified into spindle cell sarcoma (4), fibrous histiocytoma (2), pleomorphic sarcoma (3), liposarcoma (2), leiomyosarcoma (2), fibrosarcoma (1) and round cell sarcoma (1). In the remaining 3 patients immunohistochemistry studies confirmed a metastatic squamous cell sarcoma, a metastatic malignant melanoma and a metastasis from a poorly differentiated upper gastrointestinal malignancy. Lung metastases were present at the time of referral in 6 patients and developed later during follow-up in 4 patients.

For patients where curative surgery was an option, primary wide local excision (15 patients) or primary amputation (2 patients) was performed. The remaining 2 patients presented with unresectable disease due to the location and localised spread; an embolisation was performed for palliation in both cases. Revision surgery was needed in 9 patients for either a positive resection margin confirmed by histology, or a recurrence; these included 3 secondary amputations. A histologically proven recurrence occurred in 6 patients after an average of 15.8 (4 to 41) months. Local adjuvant radiotherapy was administered to 7 patients and a combination of radio–and chemotherapy was used in 2 patients for metastases. Mortality was 53% (9 patients) by the end of 36 months follow-up period.

Conclusion: Fungation in soft tissue tumours is a rare phenomenon and often a sign of locally advanced disease with a high grade nature. Patients present with either systemic spread, or have a tendency to develop metastases despite good local disease control. Primary wide local excision is difficult with a high chance of a positive margin; hence primary amputation may be better for local clearance. Tumour recurrence and revision surgery, however, is common. We report a mortality rate of > 50% at the end of 3 years from presentation to treatment.

Introduction: Radical excision and amputation are the surgical options for advanced soft tissue tumours (STS) of the limbs. The sheer size of these advanced tumours in relation to the limb makes limb sparing surgery difficult. The rate of positive margins is high and further management options are quite limited.

Patients & Methods: We have identified 17 patients (13 males, 4 females) with sarcomas with skin changes at presentation, including recurrences and primary referrals. The average age was 67 (37–83) years. 11 patients had lower limb tumours and 6 had upper limb tumours. All patients were managed either with radical excision or amputation. Post op radiotherapy and chemotherapy was used s adjuvants when appropriate. All patients were followed up with regular clinical and radiological assessments for recurrences and metastases. The results of adequacy of clearance, recurrences, metastases and overall survival are presented.

Results: The follow-up was an average of 30 (7–120) months. Two patients had primary amputations and 15 had wide excision. Four patients had distant metastases at the time of referral. Positive margins were identified in 8 of the 17 patients after primary surgery. 5 patients had a single recurrence and 3 patients had two recurrences. Eight patients needed revision surgery (3 amputations/5 wider excisions) for a positive margin or a recurrence. A total of 8 patients had metastases by 1 year. Overall disease free survival in this cohort was 20 (3–41) months.

Conclusion: We encountered a very high rate of positive margins with high morbidity which seems quite common after limb sparing surgery in fungating STS. Amputation comparatively attains better local disease control but probably does not affect the overall survival.

Introduction: Limb schwannomas are benign nerve sheath tumours. They typically present with a cystic swelling or palpable lump. They may also present with symptoms relating to the affected nerve. If the lesion is solitary there is no malignant potential. Clinically the lump will be freely mobile except in the plane of the longitudinal course of the nerve and may have a positive Tinnel’s test. Magnetic resonance imaging or ultrasound scanning are key adjuncts to diagnosis. Treatment is excision of the lump by incising the epineurium, “shelling out” the lesion and preserving unaffected nerve fascicles. We present a case series from a regional soft tissue tumour centre that shows the excellent outcome that can be achieved with these methods.

Methods: We retrospectively analysed the case notes of 16 cases of schwannoma who had surgical excision and preservation of the parent nerve. Our outcome measures were resolution of symptoms, post-operative neurological function and recurrence.

Results: Of 16 patients, 12 had no neurological deficit. 1 had motor weakness (foot drop) and 1 had residual par-aesthesia post-operatively. 2 patients declined surgery. In addition, 2 patients had persistent pain post-operatively, but at reduced levels to their pre-operative pain. There were no cases of recurrence.

Discussion: The diagnosis of schwannoma should be considered in patients with a lump associated with neurological symptoms. Following confirmation of diagnosis with appropriate radiology, surgical excision should be carried out as detailed above to minimise morbidity. This should be performed by a surgeon skilled in dealing with soft tissue tumours.

Introduction: Anterior knee pain is a very common presenting symptom. Fat pad syndrome is an uncommon and a difficult condition to manage. The diagnosis is usually reached after a period of physiotherapy and investigation to rule out the more common aetiologies of anterior knee pain.

Patients & Methods: All patients who underwent excision of the infrapatellar fat pad following a diagnosis of Fat pad syndrome are included. Each patient was evaluated to exclude patellofemoral problems and intraarticular pathologies as the cause of anterior knee pain. Each patient underwent MR imaging and all the excised specimens were sent for histological analysis.

Results: The MR imaging provided with the provisional diagnosis in all patients. All the specimens were examined by a single senior histopathologist to correlate with the provisional diagnosis. The histology confirmed Hoffa’s syndrome in 5 patients and in the remaining 15 patients a spectrum of rare diagnoses as suspected by Magnetic Resonance imaging.

The more notable conditions were two synovial sarcomas, three haemangiomas and a Giant cell tumour of the tendon sheath. All patients were treated successfully with complete excision. No recurrences were recorded at the end of 3 year follow-up and all patients were symptom free.

Conclusion: The work up of a patient with suspected infrapatellar fat pad syndrome must include MR imaging and the exact underlying pathology should be confirmed with histological analysis of the excised fat pad as the rare causes include soft tissue malignancy.

Introduction: PVNS is a benign proliferative disorder of the synovium presenting as local or diffuse variants. The condition commonly involves the knee with a slow and indolent progress.

Case series:

Presented with anterior knee pain. Examination revealed supra-patellar fullness and tenderness. MRI scan showed a suspicious soft tissue tumour. Histology confirmed PVNS after excision biopsy.

Conclusion: This case series aims to highlight the complexities in diagnosing PVNS. It should be a differential diagnosis of any kind of soft tissue problem especially around the knee. Immunohistochemistry may be useful. Multiple recurrences is a problem; adjuvant therapy may be indicated in resistant cases.

Background: The standard diagnostic approach in patients suspected of having pulmonary embolism starts with D-dimer and perfusion-ventilation lung scanning. If the resulting scan is not diagnostic, pulmonary angiography will perform.

Aims: This study evaluates the effectiveness of D-dimer as a screening tool for Pulmonary Embolism (PE) and to determine the accuracy and potential clinical utility of VQ scan in the diagnosis of pulmonary embolism in orthopaedic trauma patient.

Methods: We retrospectively reviewed 757 consecutive trauma admissions involving lower extremity and spinal injuries over 12 months and identified 20 patients with symptoms mimicking PE, without typical clinical symptoms, which underwent rotten investigations for PE (ABG, Blood test, D dimer, Chest X-ray and VQ scan).

Plasma D-dimer levels were measured as a prerequisite test in all patients before undergoing VQ scan to confirm the absence of PE. All patients had either mechanical, such as foot pumps, or chemical DVT prophylaxis.

Results: The mean age was 65 (ranging from 45–88) years, there were 11 female and 9 male. D-dimer levels in the postoperative or post traumatic period were elevated. Mean value was 3.63 (sd=2.72, range 0.60–14.34), Comparing D-dimer levels between those treated operatively (14 patients) and those treated conservatively (6 patients) we found that both groups displayed the same trend; however levels were higher following surgery.

The classical triad of dyspnoea, pleuritic chest pain and haemoptysis occurred in only 2 patients. Signs of DVT were present in 3 patients. The electrocardiographs were normal in only 4 patients, though changes particularly suggestive of PE (S1Q3T3, Right bundle branch block or right heart strains) were absent in all of the patients. Chest X-ray changes were of limited value. None showed any of the supposedly characteristic changes.

All of the patients had a VQ scan were started on chemical treatment for PE and non of them had definite PE, 12 the patients had probable PE Those patient with probable scan were not subsequently given anticoagulants owing to their medical conditions

Conclusion: The D-dimer level is never normal (< 0.5mg/l), following total trauma, and so cannot exclude a DVT or PE. The level it raises to is indistinguishable form that seen in the population with a PE and so cannot identify those patients in whom further investigation is warranted. VQ scan is not a definitive diagnosis we should rely more and more on a CT Angiography scan for the diagnosis of PE in this group of patients. This should be organised by medical team, especially with shortening of training time, to avoid wasting of resources and time which are of paramount. Requesting a D-dimer test in this population wastes time and resources and is of no benefit

Introduction: Haemorrhagic Epithelioid and Spindle Cell Haemangioma (HESCH) is a new entity that was first described by Rosenberg in 1999. We report the first instance of this tumour occurring in pelvis along with literature review.

Case Report: A 65 year-old man presented with complains of pain in left hip radiating to leg of 5 months duration. X-ray & MRI scan (Fig 1, 2, 3) of his pelvis showed lytic destructive lesion in the left inferior pubic rami. Trucut biopsy showed it as a HESCH with no evidence of malignancy (Fig. 4). He successfully underwent pre op. embolization and excision of the tumour including the ischium. His symptoms started to subside within 4 weeks of surgery and he is remaining pain free at one year follow up.

Discussion: Although endovascular tumours are relatively common in soft tissues, for them to be present in the bone is very rare. This is the first reported case of HESCH occurring in the pelvis, as previous cases tend to occur in the small bones of the hands and feet and were thought to be confined to the distal extremities. The age of this patient is also a point of interest, as he is over 2 decades older than the oldest, previously reported case. Histologicaly, a mixture of both epithelioid and spindle cells are seen which grow in a lobular pattern. As the tumour grows, it can lead to destruction of bone. It is important to note that this is a benign tumour with good prognosis.

Conclusion: This case report highlights the good prognosis associated with this form of benign tumour and that embolisation and curettage are the most effective treatment methods.

Introduction: The cause for Subungual Exostosis remains unknown, although relationships with trauma, infection and irritation have all been suggested. We report the case of a 23 year old female who developed this condition after Zadek’s procedure done for an in growing toe nail.

Case Report: A 23-year-old woman presented to us with an infected ingrowing great toenail of 1 month duration. An X-ray was performed to rule out osteomyelitis (Figures 1a and 2a), and the patient was put on oral antibiotics to control infection. 12 weeks later, the patient underwent a Zadek’s procedure with uneventful post op. recovery. However, 4 months later the patient came back with increasing pain and swelling in the operated toe. Clinically, there was a shiny nodule measuring 0.5 x 0.5cm over the dorso-medial aspect of the nail bed which was tender to touch, hard in consistency and appeared to be arising from the bone. X-ray’s confirmed it as Subungual Exostosis (Figures 1b and 2b). She successfully underwent excision of the tumour and was symptom free with no signs of recurrence at six-month follow up

Discussion: Our case highlights the fact that Subungual Exostosis may be triggered off by damage to the nail bed during surgeries for ingrowing toe nails and that extreme care must be taken during nail removal to avoid injury to the nail bed. This complication is more pronounced in the presence of pre-existing infection. Excision appears to be the treatment of choice and a biopsy report is always needed for confirmation before discharging the patient from the clinic.

Conclusion: Careful handling of nail bed is necessary during surgery and this unusual complication can be included when taking consent for Zadek’s procedure.

Background – Limb salvage surgeries for soft tissue sarcomas (STS) leads to significant amount of morbidity and fear psychosis among the affected individual. We looked into the potential complications and its implications.

Patients & Methods – Retrospective review of 96 patients over a period of 5 years from 1999 to 2004 with a minimum follow up of 8 months. Complete data of every patient was reviewed with particular emphasis on post op complications.

Results – 72 patients had STS in their lower limbs while the rest had in the upper limbs. 53 tumours were either at the level of knee or above the knee while 19 were found below the knee. The anterior compartment of the thigh was the most common site (13) with Vastus Lateralis being the commonly involved muscle. Liposarcoma was the commonest tumour (22) followed by Leiomyosarcoma (19), Fibro sarcoma (14), Synovial Sarcoma (12), Rhabdomyosarcoma (10), Histiocytoma (9) and other rare sarcomas. 61 patients had wide local excision, 17 had radical excision and 12 had marginal excision. 6 patients had palliative treatment only due to extensive metastasis (mets). 38 patients had post op radiotherapy. The average interval between presentation and definitive treatment was 28 days. 22 of the 71 patients (31 %) with no mets pre op. developed mets during follow up. The commonest problem in the post op period is inadequate skin cover which required skin grafting in 17 cases and flap cover in 3. Three of the skin grafts got necrosed due to radiotherapy. Local recurrence was the next most common complication (12 patients) along with equal number of patients with lymphaedema. Seroma/Haematoma was noted in 8 patients but none required drainage. There were 7 cases of superficial infection and one deep infection. Fixed Flexion Deformity at knee of > 10 0 was noted in 5 cases. Intractable neurological pain was noted in 3 cases involving the upper limb and one involving the lower limb. 3 developed skin rashes after radiotherapy. 2 had ulnar nerve palsy and one had foot drop. DVT occurred in 3 patients. Stump neuroma created problems in 2 cases and 2 patients ended up with below knee amputation. Another notable feature was the fear psychosis among the patients about benign swellings that were present in other parts of their body either before or after surgery which resulted in 11 surgeries but none proved to be malignant. As on 31-3-2005, 39 were dead and the rest were alive. The average life span of the patients who died from the time of confirmed diagnosis was 23 months.

Discussion – There is a plethora of complications that can occur following extensive resection of huge tumours in the extremities. This may involve sacrificing neuro-vascular structures to achieve adequate clearance. In spite of this, there is evidence of frequent local recurrences and distant metastatic spread. There is still some lack of awareness among the public about innocuous looking swellings and they present late with distant metastasis when the prognosis becomes poor.

Conclusion –

Significant complications can occur after sarcoma surgeries.

Background: To look into the incidence of lymphatic spread in Soft Tissue Sarcomas (STS) of the extremities and its relevance to the patient’s prognosis.

Patients & Methods: Retrospective review of 96 patients over a period of 5 years from 1999 to 2004 with a minimum follow up of 8 months. Complete data of every patient was reviewed with particular emphasis on lymph node and distant metastasis. All the patients were seen by one consultant and the histology reports were given by one Pathologist. 2 consultant radiologists were also involved in giving reports.

Results: There were 39 males and 57 females with an average age of 51 years. The average duration of swelling at presentation was 6 months. There was a strong family history of cancer in first degree relatives in 23 patients (24%). Liposarcoma was the commonest tumour (22) followed by Leiomyosarcoma (19), Fibro sarcoma (14), Synovial Sarcoma (12), Rhabdomyosarcoma (10), Histiocytoma (9) and other rare sarcomas. The Trojani grade of the tumours was Grade 1 = 36, Grade 2 = 39 & Grade 3 = 21. No metastasis (mets) were found during pre op. screening in 71 patients (74%) while 11 (12%) had lung mets, 9 (10 %) had lymph node involvement and 5 had liver involvement (4 %). 4 had multiple organ involvement on presentation. All except 6 patients had either wide local or radical excision of the tumour. The average interval between presentation and definitive treatment was 28 days. 9 of the patients with lymph node mets underwent nodal clearance during primary surgery. 2 turned out to be reactive hyperplasia while 7 proved to be malignant. 22 of the 71 patients (31 %) with no mets pre op. developed mets during follow up at an average duration of 11 months (11 to lungs, 8 to regional lymph nodes, 2 to liver and one to bone. 4 patients had multiple mets) In addition to this, there was local recurrence in 12 patients of whom 9 had incomplete excision during primary surgery. Of the total 15 patients who had proven lymph node mets, 5 came from Rhabdomyosarcoma, 4 from Leiomyosarcoma, 3 each from Lipo & Synovial sarcoma. The average life span in patients with lymph node involvement was 13 months in total when compared to 31 months for others. As on 31-3-2005, 39 were dead and the rest were alive. The average life span of the patients who died from the time of confirmed diagnosis was 23 months. The average life span for Rhabdomyosarcoma was 8 months, Histiocytoma was 12 months, Liposarcoma was 19 months, Leiomyosarcoma was 28 months and Synovial sarcoma was 36 months. Patients with Trojani grade 3 STS died at an average of 9 months when compared to 38 months for grade 1.

Discussion: There is a 16% spread to regional lymph nodes. It appears that lymph node involvement is indicative of micrometastatic disease elsewhere. Excision of the lymph nodes during primary surgery did not improve the life expectancy.

Conclusion:

Lymph node involvement is a poor prognostic sign

Purpose of Study – The unusual presentation of this case posed a diagnostic dilemma between a chronic haematoma and soft tissue sarcoma even after full investigation and biopsy. Salient points to differentiate between the two are discussed along with literature review.

Case Report – A 61 year old gentleman presented with sudden increase in size of an already existing swelling over the mid third of right leg associated with throbbing pain & foot drop of 4 months duration. There was no recent history of trauma or bleeding abnormalities but there was a vague history of injury to his leg during his late teens. Clinical signs showed features suggestive of malignancy with engorged veins and diffuse margins with complete foot drop (Fig 1 & 2). X-rays showed calcifications within the substance of the swelling along with proximal tibiofibular synostosis (Fig 3). MRI scan revealed a well encapsulated mass between the peroneal muscles mechanically compressing the common peroneal nerve (Fig 4). Trucut biopsy showed cholesterol clefts and areas of dystrophic calcification characteristic of chronic haematoma (Fig 5). Patient successfully underwent enucleation of the swelling along with cutaneofascial suture to obliterate the dead space leading to complete recovery of foot drop. Biopsy confirmed a Chronic Haematoma.

Discussion – Reid et al first used the term chronic expanding haematoma for haematomas that persisted and increased in size more than a month after the initiating haemorrhage. The cause of initial haemorrhage is most commonly trauma which results in displacement of skin and subcutaneous fatty tissue from more deeply located fixed fascia with formation of blood filled cysts surrounded by dense fibrous tissue. Factors in the blood-clotting cascade are said to be associated with an inflammatory reaction leading to additional bleeding from fragile capillaries and thus to additional inflammation, hence setting up a self-perpetuating process.

Although the MRI & biopsy results in this case were reassuring, the clinical scenario of sudden foot drop with increase in pain point more towards a malignant process rather than a benign condition. Some salient points to differentiate the two include that sarcoma have no history of trauma and the duration of symptoms is longer in haematoma than sarcoma. Also, sarcomas usually involve deeper structures while haematoma occur in superficial layers. It should also be noted that several soft tissue sarcoma themselves commonly reveal haemorrhagic or cystic changes. Other differential diagnosis includes myositis ossificans and tumoral calcinosis.

Conclusion – It is difficult to differentiate between chronic haematoma and soft tissue sarcoma based on clinical findings alone. X-ray and biochemical tests are always essential to rule out any fracture or bony mass but MRI is the gold standard and biopsy is the only way to rule out a malignant tumour. Surgical excision of the swelling including the fibrous pseudocapsule along with cutaneofascial suture to obliterate the dead space is the treatment of choice for chronic haematoma because aspiration of the fluid or incomplete excision could lead to recurrence, continued growth or a chronic draining sinus with or without infection.

Background: Skin grafting is one of the simplest techniques of providing skin cover following enbloc resection of soft tissue sarcomas on extremities. But many authors have questioned the tolerance of skin graft to post operative radiotherapy.

Aim: To assess the integrity of skin grafts following post operative radiotherapy for soft tissue sarcomas on extremities.

Material and methods: During the period between 1997 and 2003, 10 patients received postoperative external beam radiotherapy following excision of soft tissue sarcomas on extremities and skin grafting at this regional soft tissue sarcoma unit. Age of these patients ranged from 26 years to 92 years. Malignant fibrous histiocytoma was the commonly encountered tumour. Commonest site of resection and skin grafting was lower leg. These patients were analysed for interval between skin grafting and radiotherapy, dose, type and fractions of radiation, break in radiotherapy, adjuvant chemotherapy and effect of radiation on skin graft.

Results: One patient developed moist desquamation and two developed dry desquamation during the course of treatment. All acute skin reactions were healed within 3 weeks of completion of radiotherapy. No patients required further soft tissue reconstruction.

Conclusion: Adjuvant external beam radiotherapy can be delivered to skin-grafted areas on extremities following enbloc excision of soft tissue sarcomas without any major complications. Our experience indicates that the radiation reaction can be minimised if the graft is allowed to heal adequately prior to the initiation of radiotherapy.

Background: Skin grafting is one of the simplest techniques of providing skin cover following enbloc resection of soft tissue sarcomas on extremities. But many authors have questioned the tolerance of skin graft to post operative radiotherapy.

Aim: To assess the integrity of skin grafts following post operative radiotherapy for soft tissue sarcomas on extremities.

Material and methods: During the period between 1997 and 2003, 10 patients received postoperative external beam radiotherapy following excision of soft tissue sarcomas on extremities and skin grafting at this regional soft tissue sarcoma unit. Age of these patients ranged from 26 years to 92 years. Malignant fibrous histiocytoma was the commonly encountered tumour. Commonest site of resection and skin grafting was lower leg. These patients were retrospectively analysed for interval between skin grafting and radiotherapy, dose, type and fractions of radiation, break in radiotherapy, adjuvant chemotherapy and effect of radiation on skin graft. Results: One patient developed moist desquamation and two developed dry desquamation during the course of treatment. All acute skin reactions were healed within 3 weeks of completion of radiotherapy. No patients required further soft tissue reconstruction.

Conclusion: Adjuvant external beam radiotherapy can be delivered to skin-grafted areas on extremities following enbloc excision of soft tissue sarcomas without any major complications. Our experience indicates that the radiation reaction can be minimised if the graft is allowed to heal adequately prior to the initiation of radiotherapy.

Background: Extra skeletal osteosarcomas are rare malignancies that account for less than 1% of all soft tissue sarcomas. We describe this tumour arising in the belly of vastus medialis muscle in a 56-year-old woman.

Case report: A 56 year old, otherwise healthy teacher was referred to this regional soft tissue sarcoma unit regarding a progressively enlarging mass in her right thigh. She had noticed this swelling 12 months ago, which remained pain free. On examination there was a firm swelling on the medial side of the thigh measuring 6 cm in diameter. There was no regional lymphadinopathy. MRI scan was reported as a probable soft tissue sarcoma arising from vastus medialis. An open biopsy was reported as a sarcoma of chondroid differentiation but a specific diagnosis of extraskeletal osteosarcoma was reached only after definitive tumour resection. She was treated by wide resection, post operative radiotherapy and combined chemotherapy. At 24 months of follow up she remained tumour free.

Relevant literature is reviewed.

Background: Adult rhabdomyosarcoma is a relatively rare tumour. Good prognosis has been reported in children with multimodality of management. Because of its rarity, very little has been written about this tumour in adults especially on extremities.

Aim- To analyse the results of multimodality treatment of rhabdomyosarcoma of extremities in adults treated over a period of ten years in a UK regional centre.

Material and Methods – Between 1991 and 2002, eight patients underwent enbloc resection for rhabdomyosarcoma of extremities. There were four men and four women. Age of these patients ranged from 21 to 78years. Locations of these tumours were thigh in 5 patients, legs in 2 patients and shoulder in one. Treatment consisted of surgical resection in all patients combined with radiotherapy or chemotherapy or both. These patients were studied retrospectively for surgical and treatment details, tumour recurrence, secondary and mortality. Results are analysed in relation to histological subtype, size of the tumour (less or more than 5cm) and stage of the disease.

Conclusion: Our experience shows a significant incidence of metastatic recurrence and mortality in these patients. Major determinant of disease control (local and distant) seems to be the size of the tumour at presentation. Treatment must be individualized, but complete local excision with a tumour-free margin should be the goal. Major ablative amputation surgery was not performed.

In contrast to bony metastases, soft tissue metastases from carcinoma are rare. We reviewed all referrals to our Sarcoma Unit over an eight-year period, and found an incidence of soft tissue metastases from carcinoma of 1.4%. The most common mode of presentation was a painless soft tissue lump in a patient with an occult primary. Lung and kidney were the most frequent primary sources. Overall, prognosis was poor, with a mean survival of 9.4 months. Renal tumours however had a much better prognosis that other types of tumours. Treatment should be individualised according to the underlying disease and the prognosis. Although much rarer than primary soft tissue sarcomas, soft tissue metastases from carcinoma should remain a differential diagnosis in any patient presenting with a suspicious soft tissue lump.

Introduction: Carcinoma most commonly metastasises via the lymphatic system firstly to the regional lymph nodes and then into the general circulation. Dependent upon the primary site of tumour, metastases from carcinoma commonly occur to the lungs, liver and bone. Distant metastases to the soft tissues are rare. There are few published case series (1,2) – the majority of the literature containing only case reports. We report a series of 10 cases of soft tissue metastases from carcinoma, collected from retrospective review of the case notes of consecutive patients referred over a eight year period to our Sarcoma Unit with a soft tissue lump, suspicious of a sarcoma.

Patients and Methods: We retrospectively reviewed the case notes of consecutive patients over a eight year period (April 1995 – April 2003) referred to our Unit with a soft tissue lump, suspicious of a sarcoma. All patients underwent magnetic resonance (MR) scanning, and then trucut or open biopsy of the lesion. Dependent on the histological and MR findings, patients then underwent computer tomography (CT) of the chest and ultrasound examination of the abdomen. Included in this series were all patients with a histologically proven, soft tissue (skeletal muscle or subcutaneous tissue) metastatic carcinomatous deposit.

Demographic, diagnostic, clinical, radiological and treatment data was collected on all patients.

Results: Of the 702 referrals to our Unit over the eight-year period with a soft tissue lump suspicious of a sarcoma, 10 cases proved to be soft tissue metastases from carcinomas (incidence 1.4%). Data for the 10 patients comprising the series is shown in Table 1. Eight of the patients were male, two were female. The mean age at presentation was 68 years (range 39–85 years). Two patients presented with a painful lump, and in the other eight patients the lump was asymptomatic. The involved sites included the thigh in four cases, the arm in three cases, the back, buttock and axilla one each.In nine cases, the soft tissue lump was the presenting symptom of an occult primary carcinoma, whilst in one case (Case 4); the patient had a history of previous excision of a hypernephroma. The sources of primary carcinoma were small cell carcinoma of the lung in 4 cases, renal clear cell carcinoma in 3 cases, large bowel adenocarcinoma in 1 case, prostate 1 case, and in 1 case the primary site was unknown.

All but one patient (Case 6) underwent radiotherapy or chemotherapy or both. Case 6 presented with a soft tissue lump over the shoulder, which on biopsy was found to be metastatic adenocarcinoma of large bowel origin. CT scan of the head confirmed multiple brain metastases. He declined any treatment and died within 2 months of presentation. In total, nine of the ten patients have died of their disease. The mean duration from diagnosis of soft tissue metastasis to death was 9.4 months (range 2–31 months). The duration of survival was significantly better for metastatic carcinoma of the kidney (23 months) compared to the other carcinomas (7 months).

Discussion: The most commonly reported primary carcinomas to result in soft tissue metastases are those of the lung, kidney and colon (1–3), contrasting with those carcinomas which commonly metastasise to bone such as prostate, breast and thyroid which only very rarely metastasise to the soft tissues. Damron and Heiner (1) who reported the largest series to date however had no cases where the patients primary site of carcinoma was of renal or colon origin and suggested that these cases were over-represented in the literature. Our series differs from their findings, concurring instead with the other published literature. Histologically, the most common diagnosis is adenocarcinoma, though many have been reported (1–3).

Soft tissue metastases from carcinoma are rare, which again contrasts to bony metastases from carcinoma. Tolia and Whitmore (4) reviewed 586 patients with renal cell carcinoma, and whilst a quarter had evidence of distant metastases at presentation, no patient had soft tissue metastases. Chandler et al (5) reported on 726 patients who died following metastatic renal carcinoma; only 3 patients had soft tissue metastases, all of which were only found at autopsy. Our series, which reviewed all patients referred to our Unit over a eight year period with a soft tissue lump initially suspicious of a sarcoma, found an overall incidence of 1.4%.

Damron and Heiner (1) reported that the most common mode of presentation was a painful soft tissue lump. In our series however, the majority were painless. Whilst for most, the lump is the first sign of an occult malignancy, renal cell carcinomas tend to be different, often presenting as a solitary soft tissue deposit a few months to up to 16 years after the initial diagnosis of renal cell carcinoma has been made (1,2). Our series agreed with these findings; only in 1 case (Case 4) was there evidence of previously documented carcinoma prior to presentation with the soft tissue lump.

All patients in our series underwent pre-operative MR scans, the appearances of which were not diagnostic of metastases, though highly suggestive of malignancy. Subsequently patients underwent either Tru-cut or open biopsy which gave the definitive diagnosis. As part of the pre-operative work-up, all patients had a CT of the chest and abdominal ultrasound scanning.

Rao et al (6) reported 5 cases of soft tissue metastases from primary sarcoma, concluding that metastases in these cases were seen as late events and survival was generally poor. In our series of soft tissue metastases from carcinoma, we also found that prognosis was poor (averaging 9.4 months), especially when the primary carcinoma was lung, though the prognosis, if the primary was renal carcinoma was less bleak.

Conclusions: Although rare, soft tissue metastases from carcinoma should remain a differential diagnosis in any patient presenting with a suspicious soft tissue lump. Whilst the MR scan appearances were suggestive of malignancy, they were not diagnostic of metastases. Tru-cut or open biopsy was reliable in confirming the diagnosis of carcinoma and helpful in the detection of the possible origin of the primary. The most common primary sites were lung, kidney and bowel.