Background

Modular endoprostheses today represent a standard treatment option in the management of musculoskeletal tumors of the lower extremities. Long-term results of these reconstructions, however, are often limited by the course of the underlying disease. We therefore report our experiences in cancer patients with megaprostheses of the lower limb after a minimum of 15 years.

Aim

Preoperative serum CRP has been identified as an independent predictor in various malignancies. For osteosarcoma, however, the value of serological markers is unreliable. Aim of this study was to evaluate the prognostic power of preoperative CRP in patients with osteosarcoma.

Endoprosthetic replacement following oncological conditions has shown to be at higher risk of sceptical complications due to the use of implants of unusual size, major soft tissue loss and immunsupression.

373 patients have been treated at our institution for malignant tumours of the bone or soft tissue around the knee with a modular tumour-prostheses of the knee joint since their availability from 1978. Infection or septic complications were identified in 78 patients (20.9%).

In 15 cases of superficial wound healing disturbances with a fistula simple excision and revision of the wound was performed. In 48 cases of deep periprosthetic infections patients underwent one-stage revision with explantation of the total prosthetic material except femoral and tibial stems, extensive debridement of the wound and replantation of the disinfected prostheses throughout one operation. In 8 patients two-stage revision of the prostheses was performed, using an antibiotic impregnated cement spacer and Steinmann nails. In 5 patients amputation of the affected limb was indicated, whereas 2 patients could be treated conservatively. Out of the patients treated by one-stage revision 16 developed recurrent infection and had to undergo consecutive surgery. After two-stage surgery 4 patients showed signs of septic recurrence.

According to our results deep periprosthetic infection of tumour-prostheses primarily can be treated by one-stage revision, in recurrent infections, however, two-stage revision should be performed. We additionally suggest the use of local or pedicled muscle flaps to obtain better soft tissue coverage of the prostheses after infection.

Resection of the upper limb together with the shoulder girdle is known as forequarter amputation. In selected patients, this type of resection may have to be extended to a resection including parts of the thoracic wall in order to achieve local tumour control in advanced stages of neoplasms. Although, forequarter amputation alone is well described, reports on its combination with parts of the chest wall only consist of few case reports, while larger experiences and systematic reviews of its obstacles and complications are missing.

A series of six patients, five females and one male with, average age 58 years (range 41 to74 years), undergoing forequarter amputation with partial chest wall resection has been treated for malignancies around the shoulder at the Medical University of Vienna since 1993, thereby, to our best knowledge, presenting the largest series published to date. Patient data of all departments involved were retrospectively analysed and patients were followed-up according to standard oncological protocols. The underlying disease was malignant schwannoma in two, undifferentiated sarcoma in two, osteosarcoma in one and squamous cell sarcoma in one. A vast tumour of the shoulder and axillar space, uncontrollable pain, lymphedema, partial loss of function of the affected limb and ulceration indicated treatment.

All patients underwent radical amputation of the upper limb and all structures of the shoulder girdle including two to seven ribs. Chest wall reconstruction was achieved by a PTFE patch alone or in combination with a Stratos^®-implant. Myo-cutaneous reconstruction was performed by a pedicled (n=3) or a free myo-cutaneous forearm flap (n=3). There were no cases of peri-operative mortality. In two patients full forearm flap necrosis occurred and indicated two revisions in each with coverage by a free flap. One patient suffered vascular complications and underwent five revisions. Average follow-up was 14 months (range 5 to 35 months). Median survival was 8.5 months, with three patients still alive at the time of this investigation. Three patients died of systematic metastatic disease between 5 and 35 months after surgery. Two patients were successfully supplied with myo-electrical prostheses.

The key issues for surgical management of the resulting defect were

the restoration of mechanical chest wall stability,

Although forequarter amputation is a mutilating operation and in advanced stages of disease certainly can only temporarily limit disease progression, in selected cases, it may remain an option in vast tumours of the shoulder girdle.

Background: Endoprosthetic reconstruction has become the gold standard of treatment after the resection of tumors around the proximal femur, however, the rate of complications linked to megaprostheses is clearly higher than with standard implants. Aim of this study was to investigate the incidence and type of complications related to modular proximal femur prostheses.

Patients and Methods: By retrospective database analysis of the Vienna Tumor Registry, we evaluated the incidence of complications in 170 consecutive patients who have received a proximal femur KMFTR/GMRS at our institution between 1982 and 2007. 71 patients with an average age of 41.7 years (range 18.2–79.9 years) received the implant following the resection of a malignant bone tumor, 95 patients with an average age of 61.7 years (range 5.9–84.2 years) due to metastatic disease. The average time of follow-up was 3.5 years (SD ±4.9 years).

Results: Overall patient survival at five years was 32%. For patients with metastatic disease the overall survival was 10% at five years. Patients being treated for a primary bone tumor had an overall survival of 55% at 5 years. The overall survival of the prosthesis was 90% at two years and 72% at five years. Twenty-one patients (12.65%) suffered from dislocation after a mean time of 6.5 month (range 0.3–33 months) after surgery. Out of these, nine had to be treated by open reduction. Nine patients suffered recurrent dislocation after their first event. Patients who underwent extensive pelvic reconstruction had a significantly higher dislocation rate (33.3%) compared to patients with no or standard acetabular components (11.2%). Deep infection occurred in twelve patients (7.3%) after a mean of 39 months (range 1–166 months) after surgery. Treatment of infection was one-stage revision in eight and hip disarticulation in one patient. Two patients were successfully treated by local wound revision. One patient died of septic shock four days after surgery. Re-infection occurred in three patients. Aseptic loosing occurred in 13 patients (12.8%) after a mean time of 75.6 months (range 1–223 months) after surgery. Revision surgery was necessary in 27 patients (15.8%) with a mean time to first revision of 32 months (range 0.3–116 months). Prosthetic fracture was found in two patients. Local recurrence occurred in 14 patients (8.4%). In seven patients (4.2%) amputation was necessary.

Conclusion: Modular endoprosthesis allow excellent reconstruction of the proximal femur following tumor resection. However, the main complications, dislocation and infection, still remain considerable drawbacks.

Besides the femur and the tibia, the humerus is the third most common localisation of osteosarcoma. 78 patients with osteosarcoma of the humerus have been treated at our institution since 1934. Among these, 7 patients have been admitted before implementation of the Vienna Tumour Registry in 1968, additionally 4 patients had undergone primary surgical resection at another institution. This left 67 patients for follow-up after multi-modal therapy of humeral osteosarcoma comprising neo-adjuvant and adjuvant chemotherapy and surgical resection. (38 males and 29 females with an average age of 21.8 years, range 3.6 to 73.2 years) The subtypes of tumours observed were classic osteosarcoma in 56 patients, parostal sarcoma in 4, teleangiectatic sarcoma in 3, secondary sarcoma in 2 (one in Morbus Paget and one after radiation of a hemangioendothelioma), high-grade surface sarcoma in 1 and a humeral lesion within a multifocal osteosarcoma in 1. The localisation was foremost the proximal humerus (61) and rarely affecting the distal (5) or total bone (1). 11 patients suffered from pulmonary metastases upon primary diagnosis. In 9 cases resection alone was indicated. 9 patients underwent a resection-replantation-plasty, and in 2 patients primary amputation was performed. 46 patients were treated by resection and endoprosthetic reconstruction using ceramic prostheses (7), custom-made endoprostheses (13) or humeral HMRS modular prostheses (26). Before 1980 a non-standardised neo-adjuvant and adjuvant chemotherapy was administered in 12 patients, all patients thereafter received a chemotherapeutic regimen according to the COSS or EURAMOS-1 protocol. In 3 patients with parostal sarcoma no adjuvant therapy was indicated. The patient with multifocal osteosarcoma was treated conservatively by chemotherapy, radiation and immunotherapy by dendritic cell vaccination. The overall survival was 58% at 5 years. 23 patients died of their disease at an average of 25 months after operation (range 2 to 135 months). Average follow-up of the remaining patients was 91 months. (range 1 to 389 months). 4 patients treated before 1982 developed local tumour recurrence leading to secondary amputation in all cases, and death of disease within 12 months in 3 cases, respectively. 16 patients had to undergo one or more revisions, including secondary amputation in 2. Pulmonary metastases were observed in 15 patients, 2 patients developed skeletal metastates. After resection of metastatses, 14 patients died of disease, among them 9 patients died within 12 months after operation. Upon latest follow-up, 3 patients were alive disease-free, the patient with multifocal osteosarcoma was alive with disease 22 months after primary diagnosis. The multimodal treatment of osteosarcoma shows satisfactory oncological results. The implementation of standardised chemotherapeutic protocols has improved overall outcome.

The treatment of deep prosthetic infection in cancer patients with tumour prostheses remains the major complication to be dealt with in this population.

The Vienna Bone Tumour Registry includes information of more than 6500 patients of a period of 36 years. 145 patients with malignant proximal femoral tumors had resection and limb salvage with an uncemented Kotz modular femoral and tibial reconstruction megaprosthesis (KMFTR). There were twenty osteosarcomas, thirteen Ewing’s sarcomas, six chondrosarcomas, six plasmozytomas, three fibrosarcomas, three liposacomas and others. Thirteen patients (7 males, 6 females with an average age of 45 years, range 10 to 75 years) suffered from deep prosthetic infection within an average of 44 months after primary implantation, representing an infection rate of 8,97 percent.

Average follow-up was 109 months, range 7 to 339 months. Two patients with only mild signs of infection were treated by a conservative antibiotic regimen. Nine patients were treated by one-stage revision. One of the remaining two patients with severe infection underwent exarticulation of the hip as primary intervention, the other patient died due to general sepsis on the fourth post-operative day. Six patients showed no further signs of infection. Six patients, however, required one or more reoperations due to recurrent prosthetic infection. Among these four patients have successfully been treated by repeated one-stage revision, in two patients the prosthesis had to be removed permanently.

Deep prosthetic infection around modular tumour prostheses of the proximal femur and hip seems to be less common compared to distal femur, knee or tibia. However, the treatment of this complication has a higher failure rate due to multiply recurrent infection.

Hyperactivation of the epidermal growth factor receptor (EGFR) by gene amplification, mutation as well as overexpression is a hallmark of multiple human carcinomas. However, in recent years data have accumulated that EGFR-mediated signals might also contribute to malignant progression and therapy resistance of human sarcomas. Consequently we have investigated if human osteosarcoma cell lines (n=9) express functional EGFR and its useability as therapeutic target. Osteosarcoma cells expressed distinctly differing level of EGFR reaching in some cases high amounts. However, even low expression levels were sufficient to activate both MAPK and PI3K pathways (determined by phosphorylation of ERK1/2 and S6, respectively) following EGF exposure of serum-starved cells. The EGFR-specific inhibitor gefitinib completely blocked EGF-mediated and attenuated serum-induced downstream signal activation. While gefitinib applied as single agent demonstrated only limited growth inhibiting activity in short term experiments (72h drug exposure), it led to reduced colony formation in long term experiments in the majority of cell lines. Importantly, gefitinb sensitized EGFR-expressing osteosarcoma cell lines against chemotherapy with doxorubicin and methotrexate, while it antagonised cisplatin-induced cell death. Summarizing, our data suggest that EGFR-mediated survival signals protect human osteosarcoma cells against the cytotoxic activity of several antineoplastic drugs. Consequently, combination approaches including EGFR inhibitors in addition to chemotherapy should be evaluated for treatment of high grade osteosarcoma patients.

Introduction: Total knee arthroplasty in obese patients remains a challenge to most surgeons. Surgical complication rates as well as perioperative morbidity are higher than total knee arthroplasty in the nonobese. The purpose of this paper is to review our experience with total knee arthroplasty in superobese patients (BMI> 50).

Methods: From 1998–2005, 84 patients underwent 148 knee arthroplasties. Sixty-four patients underwent simultaneous bilateral total knee arthroplasties and 20 patients underwent unilateral knee arthroplasties. They were compared with similar group of nonobese patients who underwent knee arthroplasties during the same time period. All patients received combined regional and general anesthesia.

Results: Mean follow-up was 3.8 years (2–7). Knee society scores improved by 36 points in the superobese (pre-op 47 to 83 post-op) and by 45 points in the non-obese (pre-op 47 to 93 post-op) (p< .05). There was a greater incidence of complications in the superobese group, namely superficial wound infections and deep vein thrombosis. There was late loosening in three tibial components and instability in two patients that required revision in the superobese group. No reoperations in the nonobese group.

Conclusion: Although total knee arthroplasty may be safely performed in the superobese, it may be complicated by infection, loosening, instability, and lower knee scores.

Introduction: Little data are available about the incidence and the management of hip dislocation following the implantation of megaprosthesis of the proximal femur, which is one of the main complications following this procedure.

Material and Methods: 190 patients, who received a proximal femur KMFTR/GMRS at our institution between 1982 and 2007, were retrospectively reviewed with regard to the incidence of hip dislocation as well as the success rate of the subsequent surgical/non-surgical treatment. A proximal femur tumor endoprosthesis was used in 148 patients following the resection of a malignant tumor and in 43 patients in severe revision cases following total hip arthroplasty. The average age at the time of surgery was 48 [6a to 83a] in the tumor group and 57.3 [45a to 78a] in the revision group. All of the revision cases and 12 patients from the tumor group had additional revision cups, such as the Schoellner pedestal cup.

Results: 12.3 % (18/147) of the tumor patients and 13.9% (6/43) of the revision cases dislocated at least once. 66.7% (12/18) of the first dislocations from the tumor and 50 % (3/6) of the revision group were treated with closed reduction, the rest required surgery. All patients received an abduction cast for at least 8 weeks. 38% (7/18) of the dislocated hips of tumor group (4.8% [7/147] total) and 67% (4/6) of the revision group (9.3% [4/43] total) experienced a second dislocation. 57% (4/7) of the dislocations from the tumor and 100 % (4/4) of the revision group were treated with closed reduction. Three patients from the tumor group (2% [3/147] total) experienced a total of three dislocations and one patient four dislocations (< 1% [1/147] total). The first dislocation occurred in 88% of the cases within 5 months following surgery during activities of daily living. 82% of the second dislocations and all third dislocations occurred within 4 months of the previous dislocation. Interestingly, no significant difference was found in the rate of re-dislocation between surgical and non-surgical treatment in either group.

Discussion: Dislocation of a proximal femur tumor endoprosthesis is an early complication following surgery and continues to be a challenging condition to treat, especially in cases with extensive soft-tissue defects. Since 2000, a polyester ligament is successfully used in our institution as a reinforcement to reduce the risk of hip dislocation in proximal femur tumor endoprosthesis. Surgical and non-surgical methods to reduce the risk of hip dislocation are discussed.

Parosteal osteosarcoma is an uncommon tumour. Different methods of surgical treatment have been reported. Aim of this study was to investigate differences in outcome after biological and prosthetic reconstruction.

Since August 1969, 28 patients have been treated at our institution. Average age was 26 years, range 15 to 59 years. Patient data was retrospectively reviewed within the prospective database of the Vienna Tumour Registry. Average follow-up was 133.9 months, range 8.4 to 382.6 months. Two patients died of disease 8.4 and 81.4 months after operation, respectively, another patient died due to unrelated causes 330.4 months postoperatively. All surviving patients were followed for a minimum of 3.6 months.

Location of the lesion was the distal femur (19), proximal humerus (four), proximal tibia (three), mid-diaphyseal and proximal femur (one each). In 12 patients endoprosthetic reconstruction was indicated. Biological reconstruction was performed in 11 patients. Three patients underwent rotationplasty, two patients were amputated. Eight of 12 patients with endoprostheses have been revised, five have had multiple revisions. Causes for revision were bushing wear (four), aseptic loosening (four), infection (three) and periprosthetic fracture (one). There was no local recurrence in the endoprosthetic group. Two of 11 patients with biological reconstruction underwent revision due to pseudarthrosis and femoral fracture, respectively. There were two cases of local recurrence requiring secondary amputation. Two patients with rotationplasty underwent revision for wound healing disturbance and thrombectomy, respectively. Three patients developed lung metastases, leading to death of disease in two cases of amputation and rotationplasty. One patient with endoprosthetic reconstruction was alive 129.0 months after pulmonary metastasectomy. Functional outcome was satisfactory in all patients; there were no significant differences between patients with endoprosthetic or biological reconstruction.

Biological reconstruction showed less revisions compared to endoprostheses, however, exact preoperative planning is required to obtain clear margins of resection.

The development of local recurrence after multimodal treatment of osteosarcoma is associated with a very poor prognosis. The importance of clear surgical margins has been demonstrated in multiple studies, however up to date there are no studies defining which margin width is safe from an oncological perspective. The purpose of this retrospective analysis was to evaluate whether margin width or other surgical and tumour-related factors influence the development of local recurrence in osteosarcoma patients.

The files of 1867 consecutive patients with high-grade central osteosarcoma of the extremities, the pelvic bones and the shoulder girdle, who had achieved a complete surgical remission during combined-modality therapy on neoadjuvant Cooperative Osteosarcoma Study Group (COSS) protocols between 1986 and 2005, were reviewed. Of those, the data required were available for 1369 patients, who were the subject of this analysis. Eighty of these patients developed a local recurrence during the course of their illness.

The median surgical margin width amounted to 45 mm (range, 0 to 140 mm) in the local recurrence (LR) group and 50 mm (range, 0 to 350 mm) in the non-local recurrence (NLR) group (p=0.106). No statistically significant difference between the two groups was found regarding tumour size (mean, 10.38 cm and 9.53 cm respectively, p=0.169), T-status (p=0.225) and presence of pathological fracture (p=0.231). However infiltration of the soft tissue beyond the periosteum was documented in 58.8% of the patients with local recurrence and only in 36.9% of the rest (p=0.003). Furthermore, in 50% of the LR group the biopsy had been performed in a centre other than the one performing the definitive tumour resection, compared to 30.2% of the NRL group (p=0.001).

In conclusion, the absolute metric width of surgical margins does not define oncological safety. Local recurrence is more likely to develop in patients with soft tissue infiltration beyond the periosteum or those biopsied in a centre other than the one performing the tumour resection.

Fibula autograft reconstruction, both vascularised (v) and non-vascularised (nv), has been established as a standard method in limb salvage surgery of bone and soft tissue tumours of the extremities. This study retrospectively analyses the results of fibula autograft procedures in general and in relation to vascular reconstruction or simple bone grafting.

Since the implementation of the Vienna Tumour Registry in 1969, 26 vascularised and 27 non-vascularised fibula transfers have been performed at our institution in 53 patients, 26 males and 27 females with an average age of 21 years (range 4 to 62 years). Indications included osteosarcoma in 18, Ewing’s Sarcoma in 15, adamantinoma in 5, leiomyosarcoma in 3 and others in 12. Thirty patients were operated for reconstruction of the tibia (8v/22 nv), 7 for the femur (6v/1nv), 7 for defects of the forearm (4v/3nv), 5 for metarsal defects (all v), 3 for the humerus (1v/2nv) and one patient was treated for a pelvic defect (nv).

Average follow-up was 63 months (range 2 to 259 months). 43 patients showed successful primary bony union of the autograft. In 12 cases pseudarthrosis indicated further surgical revision, 9 of these patients were primarily reconstructed by use of a nv autograft. 4 patients, 2 with v and 2 with nv reconstruction, suffered a fracture of the transplant and were operated for secondary osteosynthesis. 10 patients with v bone graft developed wound healing disturbances which led to surgery, 2 patients with nv grafts suffered such complications. In 2 patients recurrent infection of a nv and a v fibula transfer led to the implantation of a modular tumour prostheses or amputation, retrospectively. Function of all patients with primary bone healing was rated satisfactory.

The use of fibula autograft in limb-salvage surgery under oncological conditions allows biological reconstruction with good functional outcome, especially when primary bone healing is achieved. Vascularised bone grafting seems to have a better outcome in terms of primary bone healing than simple fibula bone grafting, and thus represents a feasible choice in the reconstruction of bone defects from tumour resection.

In a randomised study, 28 patients with a mean age of 62.2 years (32 to 81) with osteoarthritis or avascular necrosis of the hip received either a ceramic-on-ceramic or a metal-on-metal total hip replacement. Apart from the liners the acetabular and femoral components were made of Ti-Al-Nb alloy. The serum aluminium and cobalt levels were measured before, and at one year after surgery.

The 15 patients in the ceramic-on-ceramic group had a median pre-operative aluminium level of 1.3 μg/l (0.25 to 8.4) and a cobalt level below the detection limit. At one year the aluminium level was 1.1 μg/l (0.25 to 2.3) and the cobalt level was 0.4 μg/l (0.15 to 0.7). The 13 patients in the metal-on-metal group had a median pre-operative aluminium level of 1.9 μg/l (0.25 to 4.4) and a cobalt level below the detection limit. At one year the median aluminium level was 0.9 μg/l (0.25 to 3.9) whereas the cobalt level was 1.4 μg/l (0.5 to 10.5). This increase in the cobalt level at one year was significant (p < 0.001).

Our findings indicate that ceramic-on-ceramic bearings do not cause elevated levels of serum aluminium in the first post-operative year.

Introduction: Transplantation of autologous chondrocites offers promising results. A new technique is now gaining ground which uses a “scaffolding” of hyaluronic acid (Hyalograft C©, Fidia, Italy).

Materials and methods: Thirty-five patients have been treated with Hyalograft C© since 2000. 31 were operated on for a knee and 4 for an ankle. Clinical progression was documented both preoperatively and postoperatively by means of a clinical protocol (VAS-Scale, Lysholm, ICRS, IKDC; AOFAS, Cincinnati). Twenty-one patients with knee lesions (11 had particular circumscribed defects, 7 had complex lesions and/or related lesions, 3 patients were given this indication as a last resort in an effort to avoid the use of a prosthesis) and 4 with ankle lesions were followed up for over 6 months postop.

Results: Assessment by means of the VAS-Scale showed a reduction in pain. In addition, it was possible to show an improvement in function ranging from 51 points pre-op to 75 points post-op on the Lysholm Score. In patients with particular lesions an improvement of 57 to 97 points was achieved. All of the 3 patients where the indication was used as a last resort received a prosthesis. In ankles, the improvement ranged between 2,5 to 6.3 points on the modified Cincinnati-Score.

Conclusions: In the case of the classical indication for isolated femoral defects, the results obtained with Hyalograft C© show similar results to I.C.A. In complex lesions, results were considerably worse. Osteoarthritis has shown itself not to be an indication for this technique. On the other hand, the use of Hyalograft C© makes it possible to perform transplants with a smaller surgical incision as well as to fill defects without resorting to sutures.

Aim: The purpose of this study was to describe the prevalence, recurrence rate and disease-free interval of aneurysmal and juvenile bone. Methods: A analysis of 141 cases of cystic bone lesions recorded in the Vienna Bone Tumor Registry between 1970 and 2000 was performed. 73 aneurysmal and 68 juvenile bone cysts were registered. 128 (92%) cases were managed by intralesional curettage and adjunctive chemical cautery with phenol followed by bone grafting. Seven cases (4%) were treated by single curettage, in four cases (2.8%) a marginal enbloc resection was performed and two (1.2%) cases were treated by intracystic injections of methylprednisolone acetate. All patients were followed up for a mean of 36 months (range, seven to 144 months) with frequent clinical and radiological examination. Results: The annual prevalence of aneurysmal bone cyst was between 0 and 1.238 per 105 individuals and for juvenile bone cyst between 0 and 0.963. The median age was 11.1 years (range 1– 19.7 years) with a male to female ratio of 1:1.81 for aneurysmal bone cyst and 10.4 years (range 0.5–19.9 years) and a rate of 1:1.96 for juvenile bone cyst. The cumulative probability of a survival without months after surgery was 0.83 (95% confidence interval, 0.77 to 0.90) for aneurysmal bone cyst and 0.79 (95% confidence interval, 0.69 to 0.90) for juvenile bone cyst. Conclusion: We think that the intralesional curettage with additional phenol induced cautery followed by bone grafting provides excellent results and is a successful and sufficient procedure in the therapy of these non-neoplastic bone lesions.

Aims: Clinical studies have revealed issues with residual particles from roughening processes on the surface of cementless stems. A new stem was therefore developed to achieve optimal primary stability in the femur but with a rough contamination-free surface for osteo-integration. Methods: TMZF, a new high strength and low modulus beta-titanium alloy was chosen for the stem to achieve optimal osseous integration. Several methods to achieve the goal of a rough and contamination free surface have been evaluated and documented by laser proþlometry, SEM and EDAX. Prototypes of the stem with different edge radii were implanted into human cadaver femora and tested with respect to their rotational stability. Early clinical experience was collected on the þrst 280 cases with currently short-term follow up. Results: With TMZF¨ a smaller neck diameter is possible, giving increased range of motion. With an improved process the required surface roughness and pattern for osteo-integration was achieved without any residual contaminants. Stems with a decreased radius of the edges of this rectangular design were less stable in the cadaver tests than those with sharp edges and the stem is manufactured accordingly. Conclusions: A straight stem (Hipstarª) with sharp edges made from a high performance titanium beta-alloy, reduced neck diameter and non contaminated rough surface, has been developed and tested in vitro and in vivo. A multicentric prospective clinical study has been initiated to conþrm the advantages of this innovative stem.

Ten year results of 100 primary lower limb reconstruction prostheses implanted between 1982 and 1989 were analysed. In a Kaplan-Meier estimate there was a 85% three years, a 79% five years and 71% ten years survival rate. Most common reason for implant failure was aseptic loosening in 27% of patients (11 patients; range 10–121 months) after initial operation. Other reasons for revision surgery were implant failure (4) and infection (4). Early repair of prostheses-related minor complications, was polyethylene bushings destruction. After a median followup of 127.5 months after the initial surgery, 51 patients had died and eight patients were lost to followup. Forty-one patients were evaluated clinically and radiologically using the MTS score and the radiologic implant evaluation system of the International Symposium on Limb Salvage. 41 patients had a mean of 80% (range, 40%–100%) of the normal functional capability.

Introduction and Objectives: Autologous chondrocyte suspension implantation (ACSI) has yielded good to excellent results in the treatment of cartilaginous defects of the knee. Thus far, studies on the ankle, analysing fewer subjects, offer promising results. Use of this technique in the ankle joint requires medial malleolar arthrotomy and osteotomy. Matrix-induced chondrocyte implantation (MICI) is a new technique involving the use of a hyaluronic acid-based matrix containing cultured chondrocytes.

Materials and Methods: Eight patients (4 male, 4 female) with an average age of 31 years (21–43) with defects in the talus were treated using ACSI and MICI. Average defect size was 1.9 cm. All patients had previously undergone surgery, and MRI showed Outerbridge grade IV osteochondral lesions on the talus. After clinical and radiological evaluation, arthroscopic surgery was performed to biopsy the articular cartilage of the talus. Later, a second surgery was performed with a mini-arthrotomy and debridement and cleaning of the defect. In ACSI, the defect is covered by suturing a periosteal graft to the cartilage, and the chondrocyte suspension is injected underneath. In MICI, a sheet of hyaluronic acid matrix with autologous chondrocytes of the same size as the defect is placed on the defect site and attached with fibrin glue. Patients were examined 28 months after implantation and evaluated using the Hannover Scoring system for the ankle.

Results: Follow-up results on the 8 patients verified an improvement of joint function and a reduction of pain in all cases. Hannover Scores increased in all patients. The osteotomy of the malleolus healed in all 8 cases. One patient was able to return to active competition in decathlon events.

Discussion and Conclusions: MICI requires a less complex surgical procedure and allows for a smaller incision. This technique therefore represents a broader application of tissue engineering in the treatment of cartilaginous defects of the ankle.

We studied the bone mineral density (BMD) of 48 long-term survivors of highly malignant osteosarcoma who had been treated according to the chemotherapy protocols of the German- Swiss-Austrian Co-operative Osteosarcoma Study Group which include high-dose methotrexate. The mean age of the patients was 31 ± 4.2 years and the mean follow-up 16 ± 2.2 years. The BMD of the lumbar spine and of the proximal femur of the non-operated side was measured by dual- energy x-ray absorptiometry. A questionnaire was given to determine life-style factors, medical history and medication. Ten patients were osteoporotic, 21 osteopenic and 17 normal according to the WHO definition.

Eighteen patients suffered fractures after receiving chemotherapy and all had significantly lower levels of BMD for all the sites measured.