In vivo bioluminescence
imaging of transplanted bone marrow mesenchymal stromal cells using
a magnetic delivery system in a rat fracture model

A. Kodama; N. Kamei; G. Kamei; W. Kongcharoensombat; S. Ohkawa; A. Nakabayashi; M. Ochi

doi:10.1302/0301-620X.94B7.28521

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In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model

A. Kodama
N. Kamei
G. Kamei
W. Kongcharoensombat
S. Ohkawa
A. Nakabayashi
M. Ochi

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In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model

Kodama A, Kamei N, Kamei G, et al. In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model. J Bone Joint Surg Br. 2012;94-B(7):998-1006. doi:10.1302/0301-620X.94B7.28521

Kodama, A., et al. “In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model.” The Journal of Bone & Joint Surgery British Volume, vol. 94-B, no. 7, 2012, pp. 998-1006., https://doi.org/10.1302/0301-620X.94B7.28521

Kodama, A., Kamei, N., Kamei, G., Kongcharoensombat, W., Ohkawa, S., Nakabayashi, A., & Ochi, M. (2012). In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model. The Journal of Bone & Joint Surgery British Volume, 94-B(7), 998-1006. https://doi.org/10.1302/0301-620X.94B7.28521

Kodama, A., Kamei, N., Kamei, G., Kongcharoensombat, W., Ohkawa, S., Nakabayashi, A. et al. (2012) “In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model.” The Journal of Bone & Joint Surgery British Volume, 94-B(7), pp. 998-1006. Available at: https://doi.org/10.1302/0301-620X.94B7.28521

Kodama, A., N. Kamei, G. Kamei, W. Kongcharoensombat, S. Ohkawa, A. Nakabayashi, and M. Ochi. “In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model.” The Journal of Bone & Joint Surgery British Volume 94-B, no. 7 (2012): 998-1006. https://doi.org/10.1302/0301-620X.94B7.28521

Kodama A, Kamei N, Kamei G, Kongcharoensombat W, Ohkawa S, Nakabayashi A, et al. In vivo bioluminescence imaging of transplanted bone marrow mesenchymal stromal cells using a magnetic delivery system in a rat fracture model. J Bone Joint Surg Br. 2012 Jul 1;94-B(7):998-1006. https://doi.org/10.1302/0301-620X.94B7.28521

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Abstract

For the treatment of ununited fractures, we developed a system of delivering magnetic labelled mesenchymal stromal cells (MSCs) using an extracorporeal magnetic device. In this study, we transplanted ferucarbotran-labelled and luciferase-positive bone marrow-derived MSCs into a non-healing femoral fracture rat model in the presence of a magnetic field. The biological fate of the transplanted MSCs was observed using luciferase-based bioluminescence imaging and we found that the number of MSC derived photons increased from day one to day three and thereafter decreased over time. The magnetic cell delivery system induced the accumulation of photons at the fracture site, while also retaining higher photon intensity from day three to week four. Furthermore, radiological and histological findings suggested improved callus formation and endochondral ossification. We therefore believe that this delivery system may be a promising option for bone regeneration.

Correspondence should be sent to Dr A. Kodama; e-mail: akira5152001@yahoo.co.jp

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