Aims

The aim of this study was to develop a psychometrically sound measure of recovery for use in patients who have suffered an open tibial fracture.

Aims

This study describes the use of the Masquelet technique to treat segmental tibial bone loss in 12 patients.

We conducted a randomised controlled trial to determine whether active intense pulsed light (IPL) is an effective treatment for patients with chronic mid-body Achilles tendinopathy. A total of 47 patients were randomly assigned to three weekly therapeutic or placebo IPL treatments. The primary outcome measure was the Victorian Institute of Sport Assessment – Achilles (VISA-A) score. Secondary outcomes were a visual analogue scale for pain (VAS) and the Lower Extremity Functional Scale (LEFS). Outcomes were recorded at baseline, six weeks and 12 weeks following treatment. Ultrasound assessment of the thickness of the tendon and neovascularisation were also recorded before and after treatment.

There was no significant difference between the groups for any of the outcome scores or ultrasound measurements by 12 weeks, showing no measurable benefit from treatment with IPL in patients with Achilles tendinopathy.

Cite this article: Bone Joint J 2013;95-B:504–9.

Acetabular fractures are amongst the most complex fractures. It has been suggested that pre-contouring the fixation plates may save intra-operative time, blood loss, reduce intra-operative fluoroscopy and improve the reduction. The purpose of this study was to assess if the contouring could be done reliably using the mirror image of the uninjured hemipelvis.

Using the CT data of 12 specimens with no bony abnormality 3D models were reconstructed. Using computer software (AMIRA, Visage Imaging) the mirror image of the left hemipelvis and the right hemipelvis were superimposed based on landmarks. The distances between the surfaces were then calculated and displayed in the form of colour maps.

The colour maps demonstrated that for the areas around were acetabular fixation plates would be placed the differences were small. For the anterior column plate 50% of the specimens had differences of less than 1mm, which based on the work of Letournel and Judet would represent an anatomical reduction. For the posterior column plate 58% had differences of less than 1mm.

This study demonstrates that there is considerable symmetry between both hemipelvises and that precontouring on the mirror image of the uninjured side is an accurate, quick and reliable method for precontouring.

Introduction

The ability of activated platelets to induce cellular proliferation is well recognised. In a previous diffusion model, platelets combined with Tri-calcium phosphate (TCP) led to an osteoprogenitor mitogenic response followed by cellular differentiation. This study was designed to look at osteoprogenitor responses when cultured directly onto TCP granules combined with activated platelets.

Acetabular fractures are amongst the most complex fractures to treat. It has been suggested that pre-contouring the fixation plates may save intra-operative time, blood loss, reduce intra-operative fluoroscopy and improve the reduction. The purpose of this study was to assess if the contouring could be done reliably using the mirror image of the uninjured hemipelvis.

Using the CT data of 12 specimens with no bony abnormality 3D models were reconstructed. Using computer software (AMIRA, Visage Imaging) the mirror image of the left hemipelvis and the right hemipelvis were superimposed based on landmarks. The distances between the surfaces were then calculated.

The results were collected in the form of mean distance and colour maps. The mean difference between surfaces ranged from 1.76mm and 8.47mm. The colour maps demonstrated that for the areas around were acetabular fixation plated would be placed the differences were small. For the anterior column plate 6 (50%) of the specimens had differences of less than 1mm, which based on the work of Letournel and Judet would represent an anatomical reduction. (None had a difference of more than 6mm.) For the posterior column plate 7 (58%) had differences of less than 1mm. (None had a difference of more than 3mm.

This study demonstrates that there is considerable symmetry between both hemipelvises and that precontouring on the mirror image of the uninjured side is an accurate, quick and reliable method for precontouring. However the symmetry is not exact and the operating surgeon needs to be aware that fine-contouring may be required intra-operatively.

Introduction

Chronic mid body Achilles tendinopathy is a common problem. There is no consensus on treatment. The aim of this review was to assess the effectiveness of physiotherapy interventions (non surgical and non pharmacological) for this condition.

Objective

To investigate the effects of trauma and fracture surgery on leukocyte maturation and function.

The treatment of femoral non-union, especially femoral exchanged nailing, has had mixed results in the recent literature. A review of the literature has suggested that exchanged nailing may be the gold standard for the treatment of femoral non-union. Is femoral exchange nailing an acceptable method of treatment of femoral fracture non-union? What is the evidence? In this systematic review we compare four different methods for the treatment of femoral non-union.

English speaking literature from 1970 to 2007 was searched using Pubmed^® and OVID™ databases and a manual reference search to reveal the original research, presenting the results of the treatment of femoral non-union with exchange nailing, plating, external fixation, and isolated bone grafting. Outcome parameters chosen were union rate and speed of union. The baseline variables chosen were age, number of surgeries, infection and the type of non-union.

In total, fifty six case series were identified containing 861 patients treated with exchange nailing (31 studies), 214 patients treated with plating (11 studies), 140 patients treated with external fixation (13 studies), 81 patients treated with bone grafting (4 studies). The average union rate was 89% for exchange nailing, 93% for plating and external fixation and 62% for bone grafting. Speed of union was 7.3 months for exchange nailing, 8.6 months for plating and 9.15 months for external fixation.

Study of baseline variables showed patients to be older in plating and external fixation groups (range 32–44 years, P< 0.001). There were more previous operations performed in the external fixation and plating groups (3 and 1.8 vs. 1.2, P< 0.001), significantly more infected non-unions in the plating and external fixation groups as compared to the exchange nailing group (40% vs. 11.5% P< 0.001) and significantly more atrophic non-unions in the plating and external fixation groups as compared to the exchange nailing group (85% vs. 65%, P< 0.001).

The literature suggests that femoral exchange nailing has an equivalent or poorer outcomes when compared to external fixation and plating in spite of having been performed in potentially less complex cases. Plating of non-union in the literature has a higher rate of associated bone grafting than femoral exchange nailing which may be responsible for marginally better union rate in the external fixation and plating groups.

Isolated bone grafting without revision of fixation does not provide adequate union rate and hence may be of questionable treatment value.

Purpose: In the modern political climate our practice is increasingly being compared with that of our peers. Outcome measures will form the basis of this. Good outcome measures have two essential requirements; they should be valid and reliable. Outcome measures are not easy to construct. Traditionally subjective walking distances have formed a portion of the assessment. This has never been validated.

Method: Null hypothesis - patients can accurately estimate their actual walking distance.

After gaining COREC approval we compared the objective to subjective walking distance of patients who had sustained a fractured Os Calcis over the past two years and were allowed to full weight bear. Patients were assessed by a senior physiotherapist and Doctor. Both the American Orthopaedic Foot and Ankle Score and Maryland Score were performed. Patients were asked to estimate their maximum walking distance prior to objective treadmill assessment.

Results: 20 patients, 6 female and 14 male were assessed. Average age was 67yrs (range 46–83yrs). One patient was excluded as they were breathless at rest. Good correlation was found between the subjective walking assessments of the two scores. All patients’ uniformly over estimated their walking ability. This was a highly significant difference, p-value 0.002. Therefore the null hypothesis has been disproved; patients’ subjective walking distance is inaccurate.

Significance: Walking distance assessment is used is as a measure in many fields of orthopaedics namely foot and ankle outcome measures and lower limb arthroplasty. This study has shown it to be an inaccurate method. It also highlights the need to validate all outcome measures.

Introduction: Elevated plasma elastase levels have been reported following major trauma and isolated femoral fracture. Reamed femoral nailing has been shown to further increase plasma elastase levels. The aim of this study was to investigate neutrophil (PMN) priming for degranulation following major trauma and isolated long-bone pelvis fracture by assessing the ability of PMN to release elastase in-vitro in response to phorbol myristate acetate (PMA) an analogue of dia-cylglycerol (DAG) a component of the “second messenger” system.

Methods: 11 major trauma (ISS≥18) patients and 18 patients with isolated long-bone/pelvis fracture, were consented to enter the study. Patients in the isolated fracture group were further stratified depending upon the type of fracture stabilization they underwent [reamed nail (n=12), Ex-Fix (n=6)]. Blood samples were obtained on admission, at 24 hours post injury, at day 3 and day 5. 11 healthy volunteers were used as controls. PMN were isolated by dextran sedimentation and ficoll-hypaque density gradient centrifugation. The ability of PMN to degranulate was assessed by an elastase substrate assay.

Results: A significant increase in the capacity of PMN to release elastase in response to a PMA stimulus was seen in major trauma patients on admission as compared with healthy volunteers. However in patients with isolated long-bone/pelvis fracture, there was no difference in levels of elastase release. Further no difference in the ability of PMN to release elastase was seen between the reamed nail and Ex-Fix groups.

Conclusions: In conclusion we show that PMN are primed for increased degranulation (elastase release) following major trauma but not following isolated long-bone/pelvis fracture. These primed PMN are capable of increased tissue damage following major trauma thus increasing the risk of development of multiple organ failure.

Aims: Neutrophil (PMN) dysfunction is implicated in both acute respiratory distress syndrome (ARDS) and sepsis. We aimed to determine the PMN response following isolated long-bone/pelvic fracture by investigating temporal changes in PMN migration and surface receptor expression (CXCR1, PECAM- 1, & CD18/ CD11b) following injury. Methods: Of the 20 patients consented to enter the study, 14 underwent reamed nailing/ORIF within 24 hours, and 6 were treated with an Ex-Fix or conservatively. 11 normal volunteers (NLV) were used as controls. Blood samples were obtained within 2 hours of admission, at 24 hours, at day 3 and day 5. PMN were isolated and the number of PMN migrating across porous collagen IV coated tissue culture inserts, in response to IL-8 were quantitated by myeloperoxidase activity. PMN surface receptor expression was assessed by whole blood FACScan analysis. Results: Signiþcantly greater numbers of fracture patient PMN migrated on admission as compared with NLV. In the Ex-Fix group the numbers migrating declined steadily and showed a hypo-response on day 5. In the reamed nailing group there was a further elevation in the PMN numbers migrating post-operatively. CXCR1 & CD18 expression was signiþcantly increased on admission. PECAM-1 was signiþcantly down-regulated on admission.

Conclusions: Following isolated long-bone/pelvic fracture PMN are primed for increased migration in response to IL-8. This is associated with up-regulation of CXCR1 and CD18, and down-regulation of PECAM-1. Treatment by reamed nailing and ORIF confers a Ç second hit È manifest as a further increase in IL-8 mediated PMN migration.

Total knee arthroplasty (TKA) is a common, effective operation but postoperative infection has devastating consequences. Several papers have associated perioperative autologous transfusion with reduced infection rates. Salvaged blood may augment the inflammatory response and central within it is polymorphonuclear leukocyte (PMN). Our hypothesis was that autologous transfusion enhances PMN activity by: increased PMN transmigration to potential infection site, enhanced phagocytosis, augmented respiratory burst activity.

Our randomised controlled prospective study showed a significant increase in superoxide production by PMN of patients who received unwashed autologous transfusion supporting the clinical studies where infection rates following autologous transfusion were reduced.

Objective: It has been observed in previous studies of autologous blood transfusion in total knee arthroplasty, that this technique is associated with a lower infection rate, though studies have not been sufficiently large to demonstrate a significant difference. We hypothesised that autologous salvage blood contained high levels of pro-inflammatory mediators which may prime or augment the patients’ inflammatory response, and, in particular, the function of the polymorphonuclear leukocyte (PMN).

Methods: Patients were randomised after consent to those receiving autologous transfusion and those having conventional treatment which was homologous transfusion only if clinically indicated. PMN were then isolated from the patients pre-admission immediately after the operation and 24 hours post-total knee arthroplasty. Three different aspects of PMN activity were measured: 1) The respiratory burst activity; 2) the ability to phagocytose microbes using radiolabelled staphylococcus epidermis; 3) and the ability to migrate across a collagen coated porous inserts.

Results: 80 patients were studied, of whom 43 were in the autologous transfusion group and 37 in the first group. Of these 37, 6 had homologous transfusion. The study showed no significant difference between the phagocytic ability of the PMN from both groups. The main stimulus to transmigration is surgery itself. There was a significant increase in the production of reactive oxygen species by the PMN of those patients who received an autologous transfusion post-operatively.

Conclusions: Using molecular biology techniques to study the PMN directly, our study indicates that autologous transfusion alters the activity of PMN and this indicates a possible mechanism whereby the immune response to infection could be enhanced. This, in turn, would explain the observed differences in infection rate in previous studies.

Background: Neutrophil (PMN) infiltration of the lung is characteristic of ARDS. Interleukin-8 (IL-8) plays a central role in the recruitment of PMN to the lung and their subsequent activation. This study examines PMN migratory activity in response to IL-8, over the first 24 hours of admissions following major trauma.

Methodology: Study Population: Adult blunt trauma victims with ISS> /=18

PMN Migraoty Activity: PMN were isolated from citrated blood at admission, 8 and 24 hours later. The number of PMN migrating across porous tissue culture inserts in response to defined concentrations of IL-8 (zero, 10, 30 & 100ng/ml) were quantitated by peroxidase assay.

Results: Significantly greater numbers of trauma patients PMN migrated to concentrations of IL-8 (30& 100ng/ml) at each time point, when compared to normal volunteers (Mann-Whitney-U Test p< 0.05). At admissions, and 8 hours later, PMN from those who later developed ARDS exhibit an enhanced migratory response to high concentrations of IL-8, in contrast to the noraml physiological attenuation of migration seen in both the remaining trauma patients (NAD) and normal volunteers (NLV).

Discussion: These data indicate that major trauma fundamentally alters the migratory capacity of circulating PMN. Within 2 hours of admission, PMN show a unique pattern of activation in those who later develop ARDS, possibly due to alteration in IL-8 receptor expression, affinity or downstream signalling. These findings suggest that limiting PMN sequestration in the lung may represent a novel therapeutic target.