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Bone & Joint Research
Vol. 8, Issue 10 | Pages 481 - 488
1 Oct 2019
Precise immunomodulation of the M1 to M2 macrophage transition enhances mesenchymal stem cell osteogenesis and differs by sex
Nathan K Lu LY Lin T Pajarinen J Jämsen E Huang J Romero-Lopez M Maruyama M Kohno Y Yao Z Goodman SB
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Objectives

Up to 10% of fractures result in undesirable outcomes, for which female sex is a risk factor. Cellular sex differences have been implicated in these different healing processes. Better understanding of the mechanisms underlying bone healing and sex differences in this process is key to improved clinical outcomes. This study utilized a macrophage–mesenchymal stem cell (MSC) coculture system to determine: 1) the precise timing of proinflammatory (M1) to anti-inflammatory (M2) macrophage transition for optimal bone formation; and 2) how such immunomodulation was affected by male versus female cocultures.

Methods

A primary murine macrophage-MSC coculture system was used to demonstrate the optimal transition time from M1 to M2 (polarized from M1 with interleukin (IL)-4) macrophages to maximize matrix mineralization in male and female MSCs. Outcome variables included Alizarin Red staining, alkaline phosphatase (ALP) activity, and osteocalcin protein secretion.

Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_2 | Pages 29 - 29
1 Jan 2016
IN VIVO HIP KINEMATICS IN HEALTHY SUBJECTS AND PATIENTS WITH OSTEOARTHRITIS DURING WEIGHT-BEARING ACTIVITIES
Hara D Nakashima Y Hamai S Higaki H Shimoto T Ikebe S Hirata M Kanazawa M Kohno Y Iwamoto Y
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Introduction

3D-to-2D model registration technique has been used for evaluating 3D kinematics from 3D surface models of the prostheses or bones and radiographic image sequences. However, no studies have employed these techniques to evaluate in vivo hip kinematics under dynamic weight-bearing conditions. The purposes of this study were to evaluate kinematics of healthy hips and also hips with osteoarthritis (OA) prior to total hip arthroplasty (THA) during four different weight-bearing activities using 3D-to-2D model-to-image registration technique.

Measurement

Dynamic hip kinematics during gait, squatting, chair-rising, and twisting were analyzed for six healthy subjects and eleven patients with osteoarthritis (OA). Continuous anteroposterior radiographic images were recorded using a flat panel X-ray detector (Fig. 1), and each hip joint was scanned by computed tomography (CT). The 3D positions and orientations of the pelvis and femur in movement cycle were determined using a 3D-to-2D model-to-image registration technique. A matching algorithm maximizing correlations between density-based digitally reconstructed radiographs from CT data and the radiographic images was applied (Fig. 2). The relative positions and orientations of the pelvis with respect to the world coordinate systems were defined as pelvic movements (anterior-posterior tilt, contralateral-ipsilateral rotation, Fig. 3b and c), and those of the femur with respect to the world coordinate systems were defined as femoral movements (flexion-extension, internal-external rotation, Fig. 3d). We also defined the relative positions and orientations of the femur for the pelvis as hip movements (flexion-extension, internal-external rotation, Fig. 3e and f).