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Orthopaedic Proceedings
Vol. 104-B, Issue SUPP_10 | Pages 59 - 59
1 Oct 2022
COMPARATIVE PHENOTYPIC AND GENOMIC FEATURES OF STAPHYLOCOCCI FROM SONICATION FLUID OF ORTHOPAEDIC IMPLANT-ASSOCIATED INFECTIONS WITH POOR OUTCOME
Santos INM Kurihara MNL Santos FF Valiatti TB d. Silva JTP Pignatari ACC Salles M
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Aim

S. aureus and S. epidermidis remain the leading biofilm-forming agents causing orthopedic implant-associated infections (OIAI), but other coagulase-negative Staphylococcus (CoNS) with clinical importance is emerging. Besides, few studies have assessed specific genomic traits associated with patient outcome. This is a preliminary descriptive study of phenotypic and genomic features identified in clinical isolates of S. aureus and CoNS isolates recovered from OIAIs patients that progressed to treatment failure.

Methods

Ten isolates were identified by matrix-time-of-flight laser-assisted desorption mass spectrometry (MALDI-TOF-MS) and tested for antibiotic susceptibility and biofilm formation. Genotypic characteristics, including, MLST (Multi Locus Sequence Typing), SCCmec typing, virulence and resistance genes were assessed by whole-genome sequencing (WGS) that was performed on an Illumina HiSeq 2500 platform. Bioinformatics analyzes were performed using CGE, PATRIC, VFDB, CARD RGI, SnapGene, BLAST, and PubMLST. S. aureus (215, 260 and 371) isolates belonged to CC5 (ST5 and ST105, spa type t002) and carried SCCmec type I (1B), II (2A) and V(5C2), respectively.