Objectives

Previously, we reported the improved transfection efficiency of a plasmid DNA-chitosan (pDNA-CS) complex using a phosphorylatable nuclear localization signal-linked nucleic kinase substrate short peptide (pNNS) conjugated to chitosan (pNNS-CS). This study investigated the effects of pNNS-CS-mediated miR-140 and interleukin-1 receptor antagonist protein (IL-1Ra) gene transfection both in rabbit chondrocytes and a cartilage defect model.

Syndactyly and polysyndactyly of the feet are common congenital conditions of the foot, and surgery to reconstruct the toes may be indicated for cosmetic, psychological, and practical reasons. A dorsal flap is traditionally used for the web space reconstruction, with skin grafts for the bases of the toes. Skin grafting has associated morbidity, and can result in pigmentation mis-match. Single-stage direct closure using a specially designed dorsal pentagonal island flap has advantages including a reduction of morbidity from avoidance of skin grafting, and shorter surgery.

Four patients with six feet were included in the study. There were two cases of syndactyly, and two cases of polysyndactyly. Bilateral involvement occurred in two patients. The average age was eighteen months at time of first surgery. Direct closure was achieved with a dorsal pentagonal island flap with dorsal and plantar triangular flaps. The average duration of follow-up was 19.8 months.

At final follow-up, all patients had acceptable web depth and pulp contour. The distance between bilateral proximal interphalangeal joints of adjacent toes, and the web slope of the reconstructed web space were compared. Complications included partial synechiae, cellulitis and keloid formation.

The dorsal pentagonal island flap is an acceptable technique in providing another means for single-stage reconstruction of the web space in syndactyly and polysyndactyly. Good functional and cosmetic outcomes can be expected. However, the potential complication of keloid formation can affect cosmesis and overall outcome, and must be understood by patients and parents.

A four-year-old boy presented with a solitary bone cyst in the odontoid process and body of the axis. Plain radiographs showed a radiolucent lesion with extreme thinning of the cortex and MRI demonstrated a high signal intensity in the interlesional matrix. The cystic component extended into the body of the axis through a defect in the epiphyseal plate.

At operation, the cavity of the cyst was found to contain serosanguineous fluid, and histological examination showed that it was lined by a thin layer of connective tissue. The cyst may have originated from a defect in the epiphyseal plate.