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Orthopaedic Proceedings
Vol. 101-B, Issue SUPP_12 | Pages 57 - 57
1 Oct 2019
PREVENTION OF PERIPROSTHETIC JOINT INFECTIONS USING ANALGESIC-LOADED UHMWPE
Gil D Grindy S Hugard S Muratoglu OK Oral E
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Introduction

Ultra-high molecular weight polyethylene (UHMWPE) can provide local sustained delivery of therapeutics1,2. For example, it can deliver analgesics to address post-arthroplasty pain2. Given that several analgesics, such as bupivacaine (anesthetic) and tolfenamic acid (NSAID), were shown to possess antibacterial activity against Staphylococci, we hypothesize that analgesic-loaded UHMWPE can also yield antimicrobial effects, preventing the development of periprosthetic joint infections.

Methods

Bupivacaine and tolfenamic acid were incorporated into UHMWPE via phase-separated compression molding. Drug release from the prepared samples was measured using high-performance liquid chromatography. Antibacterial studies of the obtained materials were conducted against methicillin-sensitive, and methicillin-resistant S. aureus, as well as S. epidermidis. Time-kill curves were obtained to characterize antimicrobial activity against planktonic bacteria. The dynamics of bacterial adhesion were assessed to characterize antibiofilm activity. Scanning electron microscopy (SEM) was used to visualize adherent bacteria. Anticolonizing activity of the tested materials was characterized using the “daughter cell” method as outlined elsewhere3. Cytotoxicity profile of drug-loaded UHMWPEs was evaluated using MG-63 osteoblast cell line.