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Orthopaedic Proceedings
Vol. 101-B, Issue SUPP_14 | Pages 26 - 26
1 Dec 2019
PHAGE THERAPY AND BACTERIAL RESERVOIRS IN BONE AND JOINT INFECTIONS: EVALUATION OF THE EFFICACY OF AN ASSEMBLY OF THREE BACTERIOPHAGES ON STAPHYLOCOCCUS AUREUS EMBEDDED IN BIOFILM OR INTERNALIZED IN OSTEOBLASTS
Kolenda C Josse J Medina M Fevre C Lustig S Ferry T Laurent F
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Aim

Staphylococcus aureus is the first causative agent of bone and joints infections (BJI). It causes difficult-to-treat infections because of its ability to form biofilms, and to be internalized and persist inside osteoblastic cells. Recently, phage therapy has emerged as a promising therapy to improve the management of chronic BJI. In the present study, we evaluated the efficacy of an assembly of three bacteriophages previously used in a clinical case report (Ferry, 2018) against S. aureus in in vitro models of biofilm and intracellular osteoblast infection.

Methods

Using HG001 S. aureus, the bactericidal activities of the assembly of the three bacteriophages (Pherecydes Pharma) used alone or in association with vancomycin or rifampicin were compared by quantifying the number of viable bacteria in mature biofilms and infected osteoblasts after 24h of exposure.