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Orthopaedic Proceedings
Vol. 103-B, Issue SUPP_15 | Pages 77 - 77
1 Dec 2021
A NOVEL ACTIVATED-ZINC ANTISEPTIC SOLUTION EFFECTIVE AGAINST STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA IN A PIG MODEL
Hill D Pinger C Noland E Morton K Hunt AA Pensler E Cantu S Attar P Siddiqi A
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Aim

Identifying the optimal agent for irrigation for periprosthetic joint infection remains challenging as there is limited data. The ideal solution should have minimal cytotoxicity while maintaining bactericidal activity. We developed a novel activated-zinc solution containing zinc-chloride (ZnCl2) and sodium-chlorite (NaClO2). The purpose of this study was 1.) to investigate the antimicrobial efficacy of 2 concentrations (“CZ1”, “CZ2”) against Staphylococcus aureus and Pseudomonas aeruginosa and 2.) to evaluate untoward effects of the solution on local wound tissue 24 hours after solution exposure in pig wound models.

Method

The study was conducted and reported in accordance to ARRIVE guidelines. We created twenty-four 1.5cm wounds on the back of a Yorkshire-cross pig. Wounds were inoculated with standardized Pseudomonas and S. aureus. 8 wounds were designated as controls (inoculum without treatment), 8 treated with CZ1, and 8 with CZ2. Punch biopsies were taken 1 hour after treatment and bacteria quantified. Wound necrosis/neutrophil infiltrate was measured 24-hours post-exposure.