Abstract
Introduction
Millions of bone graft surgeries have improved the quality of the life of millions, which cost about £ 2.5 billion annually worldwide. Current focus of the researcher is to synthesis of a bioactive bone scaffold which will reduce the cost and number of the bone graft surgeries. Hypothesis To synthesis novel, bioactive, porous, mechanical stable bone scaffold using bio glass and alginate.
Methods
Sr releasing glass was made using by melt derived technique. Composite bone scaffold was made using bio-glass and alginate. Freeze dry technique was used to make the porous bone scaffold. Bone scaffold was characterized using XRD, DSC, FTIR, Particle size analyser, SEM, ICP, Mechanical testing, Cell culture with osteoblast and fibroblast and NMR techniques.
RESULTS
Fig: Graph showing the FTIR spectra of bone scaffold.
Discussion and conclusion
FTIR shows the Si-o-Si bonds of the glass and shifting of the bonding after adding the alginate and during cross linked with Cacl2 and Srcl2. Scanning electron microscopy result shows the average pore size of around 100µm which is desirable for the growth of the cell. Release of desirable Calcium and phosphate ions can be seen from the ICP-OES measurement. Compression test allows deciding that the composite material with alginate and bioactive glass shows much better result than the simple alginate scaffolds. Cell culture showed round cells on the surface of the scaffold. And the NMR shows the interaction between bio glass and alginate. Significance We were able to produce a new novel bone scaffold which can reduce the economical burden of bone grafting.
