STRUCTURAL AND BIOMECHANICAL EVALUATION OF DECELLULARIZED HUMAN ALLOGRAFT BONE FROM DIFFERENT ANATOMICAL SOURCES

Halina Norbertczak; Hazel Fermor; Jennifer Edwards; Paul Rooney; Eileen Ingham; Anthony Herbert

doi:10.1302/1358-992X.2021.16.022

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STRUCTURAL AND BIOMECHANICAL EVALUATION OF DECELLULARIZED HUMAN ALLOGRAFT BONE FROM DIFFERENT ANATOMICAL SOURCES

The British Orthopaedic Research Society (BORS) Annual Meeting 2021, held online, 13–14 September 2021.

Halina Norbertczak
Hazel Fermor
Jennifer Edwards
Paul Rooney
Eileen Ingham
Anthony Herbert

STRUCTURAL AND BIOMECHANICAL EVALUATION OF DECELLULARIZED HUMAN ALLOGRAFT BONE FROM DIFFERENT ANATOMICAL SOURCES

Norbertczak H, Fermor H, Edwards J, Rooney P, Ingham E, Herbert A. STRUCTURAL AND BIOMECHANICAL EVALUATION OF DECELLULARIZED HUMAN ALLOGRAFT BONE FROM DIFFERENT ANATOMICAL SOURCES. Orthop Procs. 2021;103-B(SUPP_16):22-22. doi:10.1302/1358-992X.2021.16.022

Norbertczak, Halina, et al. “STRUCTURAL AND BIOMECHANICAL EVALUATION OF DECELLULARIZED HUMAN ALLOGRAFT BONE FROM DIFFERENT ANATOMICAL SOURCES.” Orthopaedic Proceedings, vol. 103-B, no. SUPP_16, 2021, pp. 22-22., https://doi.org/10.1302/1358-992X.2021.16.022

Norbertczak, H., Fermor, H., Edwards, J., Rooney, P., Ingham, E., & Herbert, A. (2021). STRUCTURAL AND BIOMECHANICAL EVALUATION OF DECELLULARIZED HUMAN ALLOGRAFT BONE FROM DIFFERENT ANATOMICAL SOURCES. Orthopaedic Proceedings, 103-B(SUPP_16), 22-22. https://doi.org/10.1302/1358-992X.2021.16.022

Norbertczak, H., Fermor, H., Edwards, J., Rooney, P., Ingham, E. and Herbert, A. (2021) “STRUCTURAL AND BIOMECHANICAL EVALUATION OF DECELLULARIZED HUMAN ALLOGRAFT BONE FROM DIFFERENT ANATOMICAL SOURCES.” Orthopaedic Proceedings, 103-B(SUPP_16), pp. 22-22. Available at: https://doi.org/10.1302/1358-992X.2021.16.022

Norbertczak, Halina, Hazel Fermor, Jennifer Edwards, Paul Rooney, Eileen Ingham, and Anthony Herbert. “STRUCTURAL AND BIOMECHANICAL EVALUATION OF DECELLULARIZED HUMAN ALLOGRAFT BONE FROM DIFFERENT ANATOMICAL SOURCES.” Orthopaedic Proceedings 103-B, no. SUPP_16 (2021): 22-22. https://doi.org/10.1302/1358-992X.2021.16.022

Norbertczak H, Fermor H, Edwards J, Rooney P, Ingham E, Herbert A. STRUCTURAL AND BIOMECHANICAL EVALUATION OF DECELLULARIZED HUMAN ALLOGRAFT BONE FROM DIFFERENT ANATOMICAL SOURCES. Orthop Procs. 2021 Dec 1;103-B(SUPP_16):22-22. https://doi.org/10.1302/1358-992X.2021.16.022

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Abstract

Introduction

Bone grafts are utilised in a range of surgical procedures, from joint replacements to treatment of bone loss resulting from cancer. Decellularised allograft bone is a regenerative, biocompatible and immunologically safe potential source of transplant bone.

Objectives

To compare the structural and biomechanical parameters of decellularised and unprocessed (cellular) trabecular bone from the human femoral head (FH) and tibial plateau (TP).

Methods

Bone pins were harvested from 10 FHs and 11 TPs (27, 34 respectively). Pins were decellularised (0.1% w/v sodium dodecyl sulphate) or retained as cellular controls. QA testing was carried out to assess protocol efficacy (total DNA and histological analysis). Cellular and decellularised FH (n=7) and TP (n=10) were uCT scanned. Material density (MD); apparent density (BV/TV); trabecular connectivity; trabecular number; trabecular thickness (Tb-t) and trabecular spacing were measured. Pins were then compression tested to determine ultimate compressive stress (UCS), Young's modulus and 0.2% proof stress.

Results

Total DNA levels of decellularised bone were below 50 ng.mg⁻¹ dry weight. Cell nuclei and marrow were largely removed. No significant differences in properties were found between decellularised and cellular bone from either anatomical region (p>0.05, Mann-Whitney). No significant differences in biomechanical properties were found between cellular FH and cellular TP (p>0.05) though significant differences in structural properties were found (MD: TP>FH, p=0.001; BV/TV: FH>TP, p=0.001; and Tb-t: FH>TP, p=0.005). Significant differences were found between decellularised FH and decellularised TP (UCS: FH>TP, p=0.001; Young's modulus: FH>TP, p=0.002; proof stress; FH>TP, p=0.001; MD: TP>FH, p<0.001; BV/TV: FH>TP, p<0.001 and Tb-t: FHT>P p<0.001.

Conclusion

Decellularisation did not affect the properties of human trabecular bone. Differences were found between the mechanical and structural properties of decellularised FH and TP which could facilitate stratified bone grafts for different applications.

Declaration of Interest

(a) fully declare any financial or other potential conflict of interest