EFFECTS OF ALPHA-TOCOPHEROL ON DISTRACTION OSTEOGENESIS

Abstract

Objective: The aim of this experimental study was to evaluate the effects of alpha-tocopherol on new bone formation by distraction osteogenesis.

Materials and Methods: Tirthy New Zealand white rabbits were randomly divided into 2 groups. Bone lengthening was performed in the right tibia through distraction osteogenesis at a rate of 0,5 mm/day for 20 days with circular external fixator (CEF). While the experimental group rabbits were administered i.m. 20mg/kg alpha tocopherol daily starting at the first day of study lasting for 20 days, control group rabbits did not receive any corresponding treatment. Radiographic examination were performed at 20th, 30th and 40th days. Scintigraphic evaluation for osteoblastic activity was at 5th and 20th day of the study. Serum total antioxidant capacity (TAC) was measured at first day and 20th day of the study. All animals were sacrificed at the end of the consolidation period of 40 days. The right tibia of all animals were removed and evaluated by histopathologic examination. Results were compared between groups and statistically analysed. A p value less than 0.05 is considered as significant in 95% confidence interval.

Results: At the 20th day, radiologic scores were statistically similar in both groups. However, at the 30th and 40th days, experimental group demonstrated statistically significant high radiologic scores and visible callus formation, maturation and remodelling. Scintigraphic baseline study at 5th day of study showed statistically similar osteoblastic activity in both groups. However, at the 20th day osteoblastic activity was significantly higher in experimental group. Serum TAC values were also significantly higher in experimental group at 20th day. At necropsy, experimental group rabbits got statistically significant high scores in histopathologic examination and showed the formation of mature bone.

Conclusions: Results of this study showed that alpha-tocopherol had beneficial effects on new bone formation by distraction osteogenesis. Administration of supplemental alpha tocopherol in patients treated with distarction osteogenesis may shorthen the framing time and increase the quality of regenerated bone.