TISSUE ENGINEERED COMPOSITES OF DEMINERALIZED BONE MATRIX AND FIBRIN GLUE

Abstract

Demineralized Bone Matrix (DBM) is currently used in various types of orthopaedic applications because of osteoconductive and osteoinductive properties. Fibrin glue is also used in cardiovascular and thoracic surgery due to its hemostatic, chemotactic and mitogenic properties. There is some possibility of being good biomaterial and biodegradable scaffold with DBM-fibrin glue mixture for bone void filler. After total hip replacement surgery, it takes long time to complete bone fusion. If patients have excess weight load after surgery, the artificial joint may not be adhered with patients’s bone.

That is why surgeons have to use any effective treatments for bone fusion for patient’s safety. In order to adapt to these surgical sites, DBMs are shaped in blocks or granules and preferable in porous forms. Combining these DBMs with fibrin glue provides a moldable and self-hardening composite biomaterial. This material will be applied to total hip replacement surgery for the effective fusion between bone and artificial joint. The aim of this work is to study the osteogenic properties of this composite material using in vivo and ex vivo. In radiological study, the DBM composite had been absorbed during one week since implantation surgery and after two weeks, some radio-opaque spots were observed in implantation sites. In histology study, Bone tissue had formed exotically in contact with the surface of the appeared well-mineralized, forming trabeculae between the granules, and had characteristics similar to those of cancellous bone. Bone growth in the tissue engineered filled with DBM and fibrin glue materials increased with implantation time.

In summary, these DBM and fibrin glue composites exhibited interesting biological and mechanical properties for filling large bone defect. These composites may be used in total hip replacement surgery for the effective filler between patient’s bone and artificial joint.