VASCULARIZATION OF THE FUSION MASS IN A EXPERIMENTAL POSTEROLATERAL INTERTRANSVERSE PROCESS FUSION WITH CORALLINE HYDROXYAPATITE AND CULTURED MESENCHIMAL STEM CELLS.

Giannicola G, Cinotti G, Riminucci M, et al. VASCULARIZATION OF THE FUSION MASS IN A EXPERIMENTAL POSTEROLATERAL INTERTRANSVERSE PROCESS FUSION WITH CORALLINE HYDROXYAPATITE AND CULTURED MESENCHIMAL STEM CELLS.. Orthop Procs. 2009;91-B(SUPP_II):269-269. doi:10.1302/0301-620X.91BSUPP_II.0910269

Giannicola, G., et al. “VASCULARIZATION OF THE FUSION MASS IN A EXPERIMENTAL POSTEROLATERAL INTERTRANSVERSE PROCESS FUSION WITH CORALLINE HYDROXYAPATITE AND CULTURED MESENCHIMAL STEM CELLS..” Orthopaedic Proceedings, vol. 91-B, no. SUPP_II, 2009, pp. 269-269., https://doi.org/10.1302/0301-620X.91BSUPP_II.0910269

Giannicola, G., Cinotti, G., Riminucci, M., Corsi, A., Ferrari, E., Mancini, U., Citro, G., Sacchetti, F., Sacchetti, B., Bianco, P., & Postacchini, F. (2009). VASCULARIZATION OF THE FUSION MASS IN A EXPERIMENTAL POSTEROLATERAL INTERTRANSVERSE PROCESS FUSION WITH CORALLINE HYDROXYAPATITE AND CULTURED MESENCHIMAL STEM CELLS.. Orthopaedic Proceedings, 91-B(SUPP_II), 269-269. https://doi.org/10.1302/0301-620X.91BSUPP_II.0910269

Giannicola, G., Cinotti, G., Riminucci, M., Corsi, A., Ferrari, E., Mancini, U. et al. (2009) “VASCULARIZATION OF THE FUSION MASS IN A EXPERIMENTAL POSTEROLATERAL INTERTRANSVERSE PROCESS FUSION WITH CORALLINE HYDROXYAPATITE AND CULTURED MESENCHIMAL STEM CELLS..” Orthopaedic Proceedings, 91-B(SUPP_II), pp. 269-269. Available at: https://doi.org/10.1302/0301-620X.91BSUPP_II.0910269

Giannicola, G., G. Cinotti, M. Riminucci, A. Corsi, E. Ferrari, U. Mancini, G. Citro, et al. “VASCULARIZATION OF THE FUSION MASS IN A EXPERIMENTAL POSTEROLATERAL INTERTRANSVERSE PROCESS FUSION WITH CORALLINE HYDROXYAPATITE AND CULTURED MESENCHIMAL STEM CELLS..” Orthopaedic Proceedings 91-B, no. SUPP_II (2009): 269-269. https://doi.org/10.1302/0301-620X.91BSUPP_II.0910269

Giannicola G, Cinotti G, Riminucci M, Corsi A, Ferrari E, Mancini U, et al. VASCULARIZATION OF THE FUSION MASS IN A EXPERIMENTAL POSTEROLATERAL INTERTRANSVERSE PROCESS FUSION WITH CORALLINE HYDROXYAPATITE AND CULTURED MESENCHIMAL STEM CELLS.. Orthop Procs. 2009 May 1;91-B(SUPP_II):269-269. https://doi.org/10.1302/0301-620X.91BSUPP_II.0910269

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Abstract

Aims: Aims of this study was to perform a quantitatively evaluation of newly formed bone, vascular density (VD) and their correlation in animal model of posterolateral spinal fusion based on skeletal stem cells (SSCs) combined with a coral.

Methods: 15 rabbits received cell-material constructs, 15 rabbits were sham-operated (decortication of transverse apophyses), 15 rabbits received material alone. After 6 months the animals were sacrified. We performed a semi-quantitative and quantitative histologycal analysis of the fusion mass. To assess the VD, sections of the fusion mass were immunolabelled for alpha-smooth muscle actin as a vascular marker.

Results: No complete fusion was observed in all groups and no bone was formed in the interapophyseal region. Aboundant newly formed bone was observed in the peri-apophyseal regions in 60% of cases. The quantitative analysis showed a significantly higher amount of bone and VD in animals treated with cells and/or biomaterial alone compared to sham (p< 0.05). Periapophyseal VD and new bone formation was significantly higher compared to interapophyseal region in all groups (p< 0.05). Positive correlation exist between newly formed bone and vascolar density (p = 0,0009).

Conclusions: Interapophyseal region is scarcely vascolarized. The study shows a positive correlation between VD and osteogenesis. The inadequacy of staminal cells could be related with the poor survival after the implant. For the use of stam cells in the APL are necessary more studies in order to clarify the survival and in situ differentiation of the grafted cells in short and mid term.

The abstracts were prepared by incoming Professor Elena Brach del Prever. Correspondence should be addressed to IORS – President office, Dipartimento di Traumatologia, Ortopedia e Mediciana del Lavoro, Centro Traumatologico Ortopedico - Via Zuretti, 29 I-10135 Torino, Italy.